Status epilepticus decreases glutamate receptor 2 mRNA and protein expression in hippocampal pyramidal cells before neuronal death

Sonja Y. Grooms, Thoralf Opitz, Michael V. L. Bennett, R. Suzanne Zukin

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104 Citations (Scopus)

Abstract

Kainic acid (KA)-induced status epilepticus in adult rats leads to delayed, selective death of pyramidal neurons in the hippocampal CA1 and CA3. Death is preceded by down-regulation of glutamate receptor 2 (GluR2) mRNA and protein [the subunit that limits Ca2+ permeability of α-amino-3-hydroxy-5- methyl-4-isoxazolepropionic acid (AMPA) receptors] in CA1 and CA3, as indicated by in situ hybridization, immunolabeling, and quantitative Western blotting. GluR1 mRNA and protein are unchanged or slightly increased before cell death. These changes could lead to formation of GluR2-lacking, Ca2+- permeable AMPA receptors and increased toxicity of endogenous glutamate. GluR2 immunolabeling is unchanged in granule cells of the dentate gyrus, which are resistant to seizure-induced death. Thus, formation of Ca2+- permeable AMPA receptors may be a critical mediator of delayed neurodegeneration after status epilepticus.

Original languageEnglish (US)
Pages (from-to)3631-3636
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume97
Issue number7
DOIs
StatePublished - Mar 28 2000

Fingerprint

AMPA Receptors
Status Epilepticus
Pyramidal Cells
Glutamate Receptors
Messenger RNA
Proteins
Kainic Acid
Dentate Gyrus
Protein Subunits
In Situ Hybridization
Glutamic Acid
Permeability
Seizures
Cell Death
Down-Regulation
Western Blotting
Acids

Keywords

  • α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors
  • Epilepsy
  • Kainate
  • Neurodegeneration
  • Seizures

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

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title = "Status epilepticus decreases glutamate receptor 2 mRNA and protein expression in hippocampal pyramidal cells before neuronal death",
abstract = "Kainic acid (KA)-induced status epilepticus in adult rats leads to delayed, selective death of pyramidal neurons in the hippocampal CA1 and CA3. Death is preceded by down-regulation of glutamate receptor 2 (GluR2) mRNA and protein [the subunit that limits Ca2+ permeability of α-amino-3-hydroxy-5- methyl-4-isoxazolepropionic acid (AMPA) receptors] in CA1 and CA3, as indicated by in situ hybridization, immunolabeling, and quantitative Western blotting. GluR1 mRNA and protein are unchanged or slightly increased before cell death. These changes could lead to formation of GluR2-lacking, Ca2+- permeable AMPA receptors and increased toxicity of endogenous glutamate. GluR2 immunolabeling is unchanged in granule cells of the dentate gyrus, which are resistant to seizure-induced death. Thus, formation of Ca2+- permeable AMPA receptors may be a critical mediator of delayed neurodegeneration after status epilepticus.",
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AU - Grooms, Sonja Y.

AU - Opitz, Thoralf

AU - Bennett, Michael V. L.

AU - Zukin, R. Suzanne

PY - 2000/3/28

Y1 - 2000/3/28

N2 - Kainic acid (KA)-induced status epilepticus in adult rats leads to delayed, selective death of pyramidal neurons in the hippocampal CA1 and CA3. Death is preceded by down-regulation of glutamate receptor 2 (GluR2) mRNA and protein [the subunit that limits Ca2+ permeability of α-amino-3-hydroxy-5- methyl-4-isoxazolepropionic acid (AMPA) receptors] in CA1 and CA3, as indicated by in situ hybridization, immunolabeling, and quantitative Western blotting. GluR1 mRNA and protein are unchanged or slightly increased before cell death. These changes could lead to formation of GluR2-lacking, Ca2+- permeable AMPA receptors and increased toxicity of endogenous glutamate. GluR2 immunolabeling is unchanged in granule cells of the dentate gyrus, which are resistant to seizure-induced death. Thus, formation of Ca2+- permeable AMPA receptors may be a critical mediator of delayed neurodegeneration after status epilepticus.

AB - Kainic acid (KA)-induced status epilepticus in adult rats leads to delayed, selective death of pyramidal neurons in the hippocampal CA1 and CA3. Death is preceded by down-regulation of glutamate receptor 2 (GluR2) mRNA and protein [the subunit that limits Ca2+ permeability of α-amino-3-hydroxy-5- methyl-4-isoxazolepropionic acid (AMPA) receptors] in CA1 and CA3, as indicated by in situ hybridization, immunolabeling, and quantitative Western blotting. GluR1 mRNA and protein are unchanged or slightly increased before cell death. These changes could lead to formation of GluR2-lacking, Ca2+- permeable AMPA receptors and increased toxicity of endogenous glutamate. GluR2 immunolabeling is unchanged in granule cells of the dentate gyrus, which are resistant to seizure-induced death. Thus, formation of Ca2+- permeable AMPA receptors may be a critical mediator of delayed neurodegeneration after status epilepticus.

KW - α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors

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KW - Kainate

KW - Neurodegeneration

KW - Seizures

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