Staphylococcus aureus-human endothelial cell interactions

Franklin D. Lowy, Jane Fant, Lore L. Higgins, Sharon K. Ogawa, Victor Bernard Hatcher

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Staphylococcus aureus is an invasive pathogen capable of causing life-threatening disease. A major component of this pathogen's virulence is its ability to invade normal endovascular tissue. We examined the interaction of S. aureus with cultured human endothelial cells and with human and rabbit endovascular tissue. Our ultrastructural study demonstrated a sequence of steps which occurred with staphylococcal invasion of human endothelial cells; adhesion, endocytosis, and intracellular replication. Ultimately, this resulted in cell disruption and death. Cytochemical staining of lysosomes demonstrated lysosomal fusion with both viable and killed intracellular bacteria without evidence of staphylococcal degradation. Quantitative studies using an in vitro infection assay demonstrated comparable rates of adhesion by viable and ultraviolet-killed bacteria, phagocytosis at a slower rate, and intracellular replication. The present study demonstrates an active role for the endothelial cell in the development and spread of endovascular staphylococcal infections. It also supports the use of this in vitro tissue culture system as a model for the study of bacterial invasion of the endothelium.

Original languageEnglish (US)
Pages (from-to)137-146
Number of pages10
JournalJournal of Ultrastructure Research and Molecular Structure Research
Volume98
Issue number2
DOIs
StatePublished - 1988

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Cell Communication
Staphylococcus aureus
Endothelial Cells
Bacteria
Staphylococcal Infections
Endocytosis
Lysosomes
Phagocytosis
Endothelium
Virulence
Cell Death
Staining and Labeling
Rabbits
Infection
In Vitro Techniques

ASJC Scopus subject areas

  • Molecular Biology
  • Anatomy

Cite this

Staphylococcus aureus-human endothelial cell interactions. / Lowy, Franklin D.; Fant, Jane; Higgins, Lore L.; Ogawa, Sharon K.; Hatcher, Victor Bernard.

In: Journal of Ultrastructure Research and Molecular Structure Research, Vol. 98, No. 2, 1988, p. 137-146.

Research output: Contribution to journalArticle

Lowy, Franklin D. ; Fant, Jane ; Higgins, Lore L. ; Ogawa, Sharon K. ; Hatcher, Victor Bernard. / Staphylococcus aureus-human endothelial cell interactions. In: Journal of Ultrastructure Research and Molecular Structure Research. 1988 ; Vol. 98, No. 2. pp. 137-146.
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