Stability of multiple antigen receptor gene rearrangements and immunophenotype in Hodgkin's disease-derived cell line L428 and variant subline L428KSA

E. S. Athan, Elisabeth M. Paietta, P. R. Papenhausen, Leonard H. Augenlicht, P. H. Wiernik, R. E. Gallagher

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Abstract

The Hodgkin's disease (HD) derived cell line L428 and a phorbol ester-selected subline L428KSA, which have been independently passaged in tissue culture for several years, were studied for possible antigen receptor gene and immunophenotypic differences. Multiple but identical alterations of these genes were found, including: the deletion of one and rearrangement of the other immunoglobulin (Ig) heavy chain allele; the rearrangement of one κ and one λ light chain allele; and the rearrangement of one T cell receptor (TCR) β allele. Restriction mapping of the Ig heavy chain locus indicated that rearrangement of the retained allele produced a J(H)-C(γ4) fusion product by an isotype switch mechanism. The 14q+ chromosome [t(14q32;?)] present in both cell cultures derived either from translocation 5' (telomeric) to the rearranged J(H) allele or 3' (centomeric) to the deleted Ig heavy chain allele and did not involve detectable rearrangement of the c-myc, bcl 1, or bcl 2 oncogenes. No differences in the immunophenotype were found between the L428 and L428KSA cells: both expressed leukocyte activation antigens and some determinants associated with myelomonocytic cells but no lymphoid markers. It is postulated that these phenotypic characteristics derived from secondary genetic events/differentiative reprogramming which produced extinction of primary lymphoid characters, including terminal deoxynucleotidyl transferase (TdT) essential to generation of the Ig and TCR gene rearrangements, and expression of an incomplete set of myelomonocytic markers.

Original languageEnglish (US)
Pages (from-to)505-510
Number of pages6
JournalLeukemia
Volume3
Issue number7
StatePublished - 1989

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Antigen Receptors
Gene Rearrangement
Hodgkin Disease
Alleles
Cell Line
Immunoglobulin Heavy Chains
T-Lymphocyte Gene Rearrangement
T-Cell Receptor Genes
Restriction Mapping
DNA Nucleotidylexotransferase
Phorbol Esters
HLA Antigens
T-Cell Antigen Receptor
Oncogenes
Genes
Immunoglobulins
Cell Culture Techniques
Chromosomes
Lymphocytes
Gene Expression

ASJC Scopus subject areas

  • Cancer Research
  • Hematology

Cite this

Stability of multiple antigen receptor gene rearrangements and immunophenotype in Hodgkin's disease-derived cell line L428 and variant subline L428KSA. / Athan, E. S.; Paietta, Elisabeth M.; Papenhausen, P. R.; Augenlicht, Leonard H.; Wiernik, P. H.; Gallagher, R. E.

In: Leukemia, Vol. 3, No. 7, 1989, p. 505-510.

Research output: Contribution to journalArticle

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AU - Wiernik, P. H.

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