Stability of a Potential Blood Substitute, HbXL99α, Under High Pressure

R. E. Hirsch, Joel M. Friedman, J. P. Harrington, S. F. Scarlata

Research output: Contribution to journalArticle

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Abstract

One important criteria for a plasma circulating hemoglobin blood substitute is resistance to subunit dissociation. For this reason, cross-linked hemoglobins (with low oxygen affinities) are being specifically designed to serve as potential blood substitutes. An example is HbXL99α, cross-linked between the α-subunits [PNAS (1987) 84:7280]. In the study presented here, the effects of up to 2 kilobars of pressure on the intrinsic fluorescence of HbXL99α, HbA and myoglobin were compared. Hemoglobin solutions were studied between 0.01 - 0.1g% in potassium phosphate or Hepes buffers, pH 7.4. Results show HbA exhibits a decrease in fluorescence intensity as a function of pressure. In contrast, HbXL99α as well myoglobin (a monomer) show essentially no significant intrinsic fluorescence changes as a function of pressure. These results suggest that HbXL99α is stable as a tetramer up to 2 kilobars of pressure. In addition, high pressure intrinsic fluorescence studies provide a suitable technique for determining the subunit stability of hemoglobins.

Original languageEnglish (US)
Pages (from-to)1635-1640
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume200
Issue number3
DOIs
StatePublished - May 15 1994

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Blood Substitutes
Hemoglobins
Pressure
Fluorescence
Myoglobin
Hemoglobin Subunits
Buffers
Monomers
Oxygen
Plasmas

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

Stability of a Potential Blood Substitute, HbXL99α, Under High Pressure. / Hirsch, R. E.; Friedman, Joel M.; Harrington, J. P.; Scarlata, S. F.

In: Biochemical and Biophysical Research Communications, Vol. 200, No. 3, 15.05.1994, p. 1635-1640.

Research output: Contribution to journalArticle

Hirsch, R. E. ; Friedman, Joel M. ; Harrington, J. P. ; Scarlata, S. F. / Stability of a Potential Blood Substitute, HbXL99α, Under High Pressure. In: Biochemical and Biophysical Research Communications. 1994 ; Vol. 200, No. 3. pp. 1635-1640.
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