Splice acceptor site for the env message of Moloney murine leukemia virus

P. A. Lazo, Vinayaka R. Prasad, P. N. Tsichlis

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We report the isolation and sequence of a cDNA clone containing part of the env message of the Moloney murine leukemia virus (MoMuLV). This clone was derived from a rat thymic lymphoma induced by MoMuLV. The AG acceptor site employed in this message is located at position 5490 in the MoMuLV genome. This splice site is detectable at the cDNA level by the creation of a novel SacI restriction site not present in the viral genome. In the -1 to -40 region, this AG acceptor site is preceded by four conserved heptanucleotides (PyXPyTPuAPy) that may function as acceptors for removal of the 5' end of the intron.

Original languageEnglish (US)
Pages (from-to)2038-2041
Number of pages4
JournalJournal of Virology
Volume61
Issue number6
StatePublished - 1987
Externally publishedYes

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Murine leukemia virus
Moloney murine leukemia virus
RNA Splice Sites
Complementary DNA
Clone Cells
clones
genome
Viral Genome
lymphoma
Introns
introns
Lymphoma
Genome
rats

ASJC Scopus subject areas

  • Immunology

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Splice acceptor site for the env message of Moloney murine leukemia virus. / Lazo, P. A.; Prasad, Vinayaka R.; Tsichlis, P. N.

In: Journal of Virology, Vol. 61, No. 6, 1987, p. 2038-2041.

Research output: Contribution to journalArticle

Lazo, P. A. ; Prasad, Vinayaka R. ; Tsichlis, P. N. / Splice acceptor site for the env message of Moloney murine leukemia virus. In: Journal of Virology. 1987 ; Vol. 61, No. 6. pp. 2038-2041.
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