Spatiotemporal dynamics of RhoA activity in migrating cells

Olivier Pertz, Louis Hodgson, Richard L. Klemke, Klaus M. Hahn

Research output: Contribution to journalArticle

521 Citations (Scopus)

Abstract

Rho family GTPases regulate the actin and adhesion dynamics that control cell migration. Current models postulate that Rac promotes membrane protrusion at the leading edge and that RhoA regulates contractility in the cell body1,2. However, there is evidence that RhoA also regulates membrane protrusion3,4. Here we use a fluorescent biosensor, based on a novel design preserving reversible membrane interactions, to visualize the spatiotemporal dynamics of RhoA activity during cell migration. In randomly migrating cells, RhoA activity is concentrated in a sharp band directly at the edge of protrusions. It is observed sporadically in retracting tails, and is low in the cell body. RhoA activity is also associated with peripheral ruffles and pinocytic vesicles, but not with dorsal ruffles induced by platelet-derived growth factor (PDGF). In contrast to randomly migrating cells, PDGF-induced membrane protrusions have low RhoA activity, potentially because PDGF strongly activates Rac, which has previously been shown to antagonize RhoA activity5,6. Our data therefore show that different extracellular cues induce distinct patterns of RhoA signalling during membrane protrusion.

Original languageEnglish (US)
Pages (from-to)1069-1072
Number of pages4
JournalNature
Volume440
Issue number7087
DOIs
StatePublished - Apr 20 2006
Externally publishedYes

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Platelet-Derived Growth Factor
Membranes
Cell Movement
rho GTP-Binding Proteins
Biosensing Techniques
Cues
Actins

ASJC Scopus subject areas

  • General

Cite this

Spatiotemporal dynamics of RhoA activity in migrating cells. / Pertz, Olivier; Hodgson, Louis; Klemke, Richard L.; Hahn, Klaus M.

In: Nature, Vol. 440, No. 7087, 20.04.2006, p. 1069-1072.

Research output: Contribution to journalArticle

Pertz, O, Hodgson, L, Klemke, RL & Hahn, KM 2006, 'Spatiotemporal dynamics of RhoA activity in migrating cells', Nature, vol. 440, no. 7087, pp. 1069-1072. https://doi.org/10.1038/nature04665
Pertz, Olivier ; Hodgson, Louis ; Klemke, Richard L. ; Hahn, Klaus M. / Spatiotemporal dynamics of RhoA activity in migrating cells. In: Nature. 2006 ; Vol. 440, No. 7087. pp. 1069-1072.
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