Sorting of β-actin mRNA and protein to neurites and growth cones in culture

Gary J. Bassell, Honglai Zhang, Anne L. Byrd, Andrea M. Femino, Robert H. Singer, Krishan L. Taneja, Lawrence M. Lifshitz, Ira M. Herman, Kenneth S. Kosik

Research output: Contribution to journalArticle

344 Scopus citations

Abstract

The transport of mRNAs into developing dendrites and axons may be a basic mechanism to localize cytoskeletal proteins to growth cones and influence microfilament organization. Using isoform-specific antibodies and probes for in situ hybridization, we observed distinct localization patterns for β- and γ-actin within cultured cerebrocortical neurons. β-Actin protein was highly enriched within growth cones and filopodia, in contrast to γ-actin protein, which was distributed uniformly throughout the cell. β- Actin protein also was shown to be peripherally localized after transfection of β-actin cDNA bearing an epitope tag. β-Actin mRNAs were localized more frequently to neuronal processes and growth cones, unlike γ-actin mRNAs, which were restricted to the cell body. The rapid localization of β-actin mRNA, but not γ-actin mRNA, into processes and growth cones could be induced by dibutyryl cAMP treatment. Using high-resolution in situ hybridization and image-processing methods, we showed that the distribution of β-actin mRNA within growth cones was statistically nonrandom and demonstrated an association with microtubules. β-Actin mRNAs were detected within minor neurites, axonal processes, and growth cones in the form of spatially distinct granules that colocalized with translational components. Ultrastructural analysis revealed polyribosomes within growth cones that colocalized with cytoskeletal filaments. The transport of β-actin mRNA into developing neurites may be a sequence-specific mechanism to synthesize cytoskeletal proteins directly within processes and growth cones and would provide an additional means to deliver cytoskeletal proteins over long distances.

Original languageEnglish (US)
Pages (from-to)251-265
Number of pages15
JournalJournal of Neuroscience
Volume18
Issue number1
DOIs
StatePublished - 1998

Keywords

  • Actin isoforms
  • Axonal transport
  • Cytoskeleton
  • Growth cones
  • In situ hybridization
  • mRNA localization

ASJC Scopus subject areas

  • Neuroscience(all)

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    Bassell, G. J., Zhang, H., Byrd, A. L., Femino, A. M., Singer, R. H., Taneja, K. L., Lifshitz, L. M., Herman, I. M., & Kosik, K. S. (1998). Sorting of β-actin mRNA and protein to neurites and growth cones in culture. Journal of Neuroscience, 18(1), 251-265. https://doi.org/10.1523/jneurosci.18-01-00251.1998