Abstract
Aging may be explained, to some extent, as a stochastic process of macromolecular damage. The rate of such a process should then determine longevity and be genetically controlled, as can be derived from the species specificity of maximum lifespan. The genome of the somatic cell is a major candidate to study for loss of DNA sequence integrity during aging. Unforturnately, a lack of adequate techniques has thus far hampered progress in testing the aging genome for changes in its DNA sequence content. Here we discuss recently developed sophisticated technology for studying spontaneous somatic mutations in relation to aging. More specifically, we described the use of a novel two-dimensional DNA typing technique for the analysis of fibroblast clones derived from primary cultures established from skin bipsies of rats different ages. Preliminary data are presented indicating the occurrence of DNA sequence changes in mini- and microsatellite regions of the rat genome at an average frequency of 2.7 × 10-3 per analyzed DNA fragment. Age-related variations in the somatic mutation frequency of these genomic regions were not observed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 311-321 |
| Number of pages | 11 |
| Journal | Mutation Research DNAging |
| Volume | 256 |
| Issue number | 2-6 |
| DOIs | |
| State | Published - 1991 |
| Externally published | Yes |
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Keywords
- Cellular ageing
- Genetic instability
- Rat fibroblast clones
- Somatic mutations
- Two-dimensional DNA typing
ASJC Scopus subject areas
- Aging
- Genetics
- Molecular Biology
Cite this
Somatic mutations and cellular aging : two-dimensional DNA typing of rat fibroblast clones. / Slagboom, P. E.; Mullaart, E.; Droog, S.; Vijg, Jan.
In: Mutation Research DNAging, Vol. 256, No. 2-6, 1991, p. 311-321.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Somatic mutations and cellular aging
T2 - two-dimensional DNA typing of rat fibroblast clones
AU - Slagboom, P. E.
AU - Mullaart, E.
AU - Droog, S.
AU - Vijg, Jan
PY - 1991
Y1 - 1991
N2 - Aging may be explained, to some extent, as a stochastic process of macromolecular damage. The rate of such a process should then determine longevity and be genetically controlled, as can be derived from the species specificity of maximum lifespan. The genome of the somatic cell is a major candidate to study for loss of DNA sequence integrity during aging. Unforturnately, a lack of adequate techniques has thus far hampered progress in testing the aging genome for changes in its DNA sequence content. Here we discuss recently developed sophisticated technology for studying spontaneous somatic mutations in relation to aging. More specifically, we described the use of a novel two-dimensional DNA typing technique for the analysis of fibroblast clones derived from primary cultures established from skin bipsies of rats different ages. Preliminary data are presented indicating the occurrence of DNA sequence changes in mini- and microsatellite regions of the rat genome at an average frequency of 2.7 × 10-3 per analyzed DNA fragment. Age-related variations in the somatic mutation frequency of these genomic regions were not observed.
AB - Aging may be explained, to some extent, as a stochastic process of macromolecular damage. The rate of such a process should then determine longevity and be genetically controlled, as can be derived from the species specificity of maximum lifespan. The genome of the somatic cell is a major candidate to study for loss of DNA sequence integrity during aging. Unforturnately, a lack of adequate techniques has thus far hampered progress in testing the aging genome for changes in its DNA sequence content. Here we discuss recently developed sophisticated technology for studying spontaneous somatic mutations in relation to aging. More specifically, we described the use of a novel two-dimensional DNA typing technique for the analysis of fibroblast clones derived from primary cultures established from skin bipsies of rats different ages. Preliminary data are presented indicating the occurrence of DNA sequence changes in mini- and microsatellite regions of the rat genome at an average frequency of 2.7 × 10-3 per analyzed DNA fragment. Age-related variations in the somatic mutation frequency of these genomic regions were not observed.
KW - Cellular ageing
KW - Genetic instability
KW - Rat fibroblast clones
KW - Somatic mutations
KW - Two-dimensional DNA typing
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UR - http://www.scopus.com/inward/citedby.url?scp=0025719344&partnerID=8YFLogxK
U2 - 10.1016/0921-8734(91)90022-4
DO - 10.1016/0921-8734(91)90022-4
M3 - Article
VL - 256
SP - 311
EP - 321
JO - Mutation Research - DNAging Genetic Instability and Aging
JF - Mutation Research - DNAging Genetic Instability and Aging
SN - 0921-8734
IS - 2-6
ER -