Somatic mutations and cellular aging

two-dimensional DNA typing of rat fibroblast clones

P. E. Slagboom, E. Mullaart, S. Droog, Jan Vijg

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Aging may be explained, to some extent, as a stochastic process of macromolecular damage. The rate of such a process should then determine longevity and be genetically controlled, as can be derived from the species specificity of maximum lifespan. The genome of the somatic cell is a major candidate to study for loss of DNA sequence integrity during aging. Unforturnately, a lack of adequate techniques has thus far hampered progress in testing the aging genome for changes in its DNA sequence content. Here we discuss recently developed sophisticated technology for studying spontaneous somatic mutations in relation to aging. More specifically, we described the use of a novel two-dimensional DNA typing technique for the analysis of fibroblast clones derived from primary cultures established from skin bipsies of rats different ages. Preliminary data are presented indicating the occurrence of DNA sequence changes in mini- and microsatellite regions of the rat genome at an average frequency of 2.7 × 10-3 per analyzed DNA fragment. Age-related variations in the somatic mutation frequency of these genomic regions were not observed.

Original languageEnglish (US)
Pages (from-to)311-321
Number of pages11
JournalMutation Research DNAging
Volume256
Issue number2-6
DOIs
StatePublished - 1991
Externally publishedYes

Fingerprint

DNA Fingerprinting
Cell Aging
Clone Cells
Fibroblasts
Genome
Mutation
Stochastic Processes
Species Specificity
Minisatellite Repeats
Mutation Rate
Microsatellite Repeats
Technology
Skin
DNA

Keywords

  • Cellular ageing
  • Genetic instability
  • Rat fibroblast clones
  • Somatic mutations
  • Two-dimensional DNA typing

ASJC Scopus subject areas

  • Aging
  • Genetics
  • Molecular Biology

Cite this

Somatic mutations and cellular aging : two-dimensional DNA typing of rat fibroblast clones. / Slagboom, P. E.; Mullaart, E.; Droog, S.; Vijg, Jan.

In: Mutation Research DNAging, Vol. 256, No. 2-6, 1991, p. 311-321.

Research output: Contribution to journalArticle

Slagboom, P. E. ; Mullaart, E. ; Droog, S. ; Vijg, Jan. / Somatic mutations and cellular aging : two-dimensional DNA typing of rat fibroblast clones. In: Mutation Research DNAging. 1991 ; Vol. 256, No. 2-6. pp. 311-321.
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