Somatic cell hybrids between Friend erythroleukemia cells and mouse hepatoma cells

Jean François Conscience; Frank H. Ruddle; Arthur Skoultchi; Gretchen J. Darlington

doi:10.1007/BF01551812

Somatic cell hybrids between Friend erythroleukemia cells and mouse hepatoma cells

Jean François Conscience, Frank H. Ruddle, Arthur Skoultchi, Gretchen J. Darlington

Cell Biology

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Somatic cell hybrids between hepatoma and Friend erythroleukemia parental cells were studied for the expression of liver-specific and erythroid properties. Several independent clones were isolated using HAT selection and were shown to be true hybrids by isozyme and chromosome analysis. All displayed a complete extinction of hemoglobin and globin mRNA production, but a retention of albumin and transferrin secretion. The data suggest that erythroid differentiation is being actively inhibited by the hepatoma genome. Possible mechanisms that might explain these results are discussed in the light of current hypotheses regarding the mechanism of cell differentiation.

Original language	English (US)
Pages (from-to)	157-172
Number of pages	16
Journal	Somatic Cell Genetics
Volume	3
Issue number	2
DOIs	https://doi.org/10.1007/BF01551812
State	Published - Mar 1977

ASJC Scopus subject areas

Genetics

Access to Document

10.1007/BF01551812

Cite this

@article{9161822f5d954857bd2278eb18688259,

title = "Somatic cell hybrids between Friend erythroleukemia cells and mouse hepatoma cells",

abstract = "Somatic cell hybrids between hepatoma and Friend erythroleukemia parental cells were studied for the expression of liver-specific and erythroid properties. Several independent clones were isolated using HAT selection and were shown to be true hybrids by isozyme and chromosome analysis. All displayed a complete extinction of hemoglobin and globin mRNA production, but a retention of albumin and transferrin secretion. The data suggest that erythroid differentiation is being actively inhibited by the hepatoma genome. Possible mechanisms that might explain these results are discussed in the light of current hypotheses regarding the mechanism of cell differentiation.",

author = "Conscience, {Jean Fran{\c c}ois} and Ruddle, {Frank H.} and Arthur Skoultchi and Darlington, {Gretchen J.}",

year = "1977",

month = mar,

doi = "10.1007/BF01551812",

language = "English (US)",

volume = "3",

pages = "157--172",

journal = "Somatic Cell Genetics",

issn = "0098-0366",

publisher = "Plenum Publishers",

number = "2",

}

TY - JOUR

T1 - Somatic cell hybrids between Friend erythroleukemia cells and mouse hepatoma cells

AU - Conscience, Jean François

AU - Ruddle, Frank H.

AU - Skoultchi, Arthur

AU - Darlington, Gretchen J.

PY - 1977/3

Y1 - 1977/3

N2 - Somatic cell hybrids between hepatoma and Friend erythroleukemia parental cells were studied for the expression of liver-specific and erythroid properties. Several independent clones were isolated using HAT selection and were shown to be true hybrids by isozyme and chromosome analysis. All displayed a complete extinction of hemoglobin and globin mRNA production, but a retention of albumin and transferrin secretion. The data suggest that erythroid differentiation is being actively inhibited by the hepatoma genome. Possible mechanisms that might explain these results are discussed in the light of current hypotheses regarding the mechanism of cell differentiation.

AB - Somatic cell hybrids between hepatoma and Friend erythroleukemia parental cells were studied for the expression of liver-specific and erythroid properties. Several independent clones were isolated using HAT selection and were shown to be true hybrids by isozyme and chromosome analysis. All displayed a complete extinction of hemoglobin and globin mRNA production, but a retention of albumin and transferrin secretion. The data suggest that erythroid differentiation is being actively inhibited by the hepatoma genome. Possible mechanisms that might explain these results are discussed in the light of current hypotheses regarding the mechanism of cell differentiation.

UR - http://www.scopus.com/inward/record.url?scp=0017377537&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017377537&partnerID=8YFLogxK

U2 - 10.1007/BF01551812

DO - 10.1007/BF01551812

M3 - Article

C2 - 204066

AN - SCOPUS:0017377537

SN - 0098-0366

VL - 3

SP - 157

EP - 172

JO - Somatic Cell Genetics

JF - Somatic Cell Genetics

IS - 2

ER -

Somatic cell hybrids between Friend erythroleukemia cells and mouse hepatoma cells

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this