Abstract
Somatic cell hybrids between hepatoma and Friend erythroleukemia parental cells were studied for the expression of liver-specific and erythroid properties. Several independent clones were isolated using HAT selection and were shown to be true hybrids by isozyme and chromosome analysis. All displayed a complete extinction of hemoglobin and globin mRNA production, but a retention of albumin and transferrin secretion. The data suggest that erythroid differentiation is being actively inhibited by the hepatoma genome. Possible mechanisms that might explain these results are discussed in the light of current hypotheses regarding the mechanism of cell differentiation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 157-172 |
| Number of pages | 16 |
| Journal | Somatic Cell Genetics |
| Volume | 3 |
| Issue number | 2 |
| DOIs | |
| State | Published - Mar 1977 |
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ASJC Scopus subject areas
- Genetics
- Medicine(all)
Cite this
Somatic cell hybrids between Friend erythroleukemia cells and mouse hepatoma cells. / Conscience, Jean François; Ruddle, Frank H.; Skoultchi, Arthur I.; Darlington, Gretchen J.
In: Somatic Cell Genetics, Vol. 3, No. 2, 03.1977, p. 157-172.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Somatic cell hybrids between Friend erythroleukemia cells and mouse hepatoma cells
AU - Conscience, Jean François
AU - Ruddle, Frank H.
AU - Skoultchi, Arthur I.
AU - Darlington, Gretchen J.
PY - 1977/3
Y1 - 1977/3
N2 - Somatic cell hybrids between hepatoma and Friend erythroleukemia parental cells were studied for the expression of liver-specific and erythroid properties. Several independent clones were isolated using HAT selection and were shown to be true hybrids by isozyme and chromosome analysis. All displayed a complete extinction of hemoglobin and globin mRNA production, but a retention of albumin and transferrin secretion. The data suggest that erythroid differentiation is being actively inhibited by the hepatoma genome. Possible mechanisms that might explain these results are discussed in the light of current hypotheses regarding the mechanism of cell differentiation.
AB - Somatic cell hybrids between hepatoma and Friend erythroleukemia parental cells were studied for the expression of liver-specific and erythroid properties. Several independent clones were isolated using HAT selection and were shown to be true hybrids by isozyme and chromosome analysis. All displayed a complete extinction of hemoglobin and globin mRNA production, but a retention of albumin and transferrin secretion. The data suggest that erythroid differentiation is being actively inhibited by the hepatoma genome. Possible mechanisms that might explain these results are discussed in the light of current hypotheses regarding the mechanism of cell differentiation.
UR - http://www.scopus.com/inward/record.url?scp=0017377537&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0017377537&partnerID=8YFLogxK
U2 - 10.1007/BF01551812
DO - 10.1007/BF01551812
M3 - Article
C2 - 204066
AN - SCOPUS:0017377537
VL - 3
SP - 157
EP - 172
JO - Somatic Cell and Molecular Genetics
JF - Somatic Cell and Molecular Genetics
SN - 0740-7750
IS - 2
ER -