Somatic cell hybrids between Friend erythroleukemia cells and mouse hepatoma cells

Jean François Conscience; Frank H. Ruddle; Arthur Skoultchi; Gretchen J. Darlington

doi:10.1007/BF01551812

Somatic cell hybrids between Friend erythroleukemia cells and mouse hepatoma cells

Jean François Conscience, Frank H. Ruddle, Arthur Skoultchi, Gretchen J. Darlington

Cell Biology

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Somatic cell hybrids between hepatoma and Friend erythroleukemia parental cells were studied for the expression of liver-specific and erythroid properties. Several independent clones were isolated using HAT selection and were shown to be true hybrids by isozyme and chromosome analysis. All displayed a complete extinction of hemoglobin and globin mRNA production, but a retention of albumin and transferrin secretion. The data suggest that erythroid differentiation is being actively inhibited by the hepatoma genome. Possible mechanisms that might explain these results are discussed in the light of current hypotheses regarding the mechanism of cell differentiation.

Original language	English (US)
Pages (from-to)	157-172
Number of pages	16
Journal	Somatic Cell Genetics
Volume	3
Issue number	2
DOIs	https://doi.org/10.1007/BF01551812
State	Published - Mar 1 1977

ASJC Scopus subject areas

Genetics

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abstract = "Somatic cell hybrids between hepatoma and Friend erythroleukemia parental cells were studied for the expression of liver-specific and erythroid properties. Several independent clones were isolated using HAT selection and were shown to be true hybrids by isozyme and chromosome analysis. All displayed a complete extinction of hemoglobin and globin mRNA production, but a retention of albumin and transferrin secretion. The data suggest that erythroid differentiation is being actively inhibited by the hepatoma genome. Possible mechanisms that might explain these results are discussed in the light of current hypotheses regarding the mechanism of cell differentiation.",

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