Somatic cell hybrids between Friend erythroleukemia cells and mouse hepatoma cells

Jean François Conscience, Frank H. Ruddle, Arthur I. Skoultchi, Gretchen J. Darlington

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Somatic cell hybrids between hepatoma and Friend erythroleukemia parental cells were studied for the expression of liver-specific and erythroid properties. Several independent clones were isolated using HAT selection and were shown to be true hybrids by isozyme and chromosome analysis. All displayed a complete extinction of hemoglobin and globin mRNA production, but a retention of albumin and transferrin secretion. The data suggest that erythroid differentiation is being actively inhibited by the hepatoma genome. Possible mechanisms that might explain these results are discussed in the light of current hypotheses regarding the mechanism of cell differentiation.

Original languageEnglish (US)
Pages (from-to)157-172
Number of pages16
JournalSomatic Cell Genetics
Volume3
Issue number2
DOIs
StatePublished - Mar 1977

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Leukemia, Erythroblastic, Acute
Hybrid Cells
Hepatocellular Carcinoma
Globins
Transferrin
Isoenzymes
Cell Differentiation
Albumins
Hemoglobins
Clone Cells
Chromosomes
Genome
Messenger RNA
Liver

ASJC Scopus subject areas

  • Genetics
  • Medicine(all)

Cite this

Somatic cell hybrids between Friend erythroleukemia cells and mouse hepatoma cells. / Conscience, Jean François; Ruddle, Frank H.; Skoultchi, Arthur I.; Darlington, Gretchen J.

In: Somatic Cell Genetics, Vol. 3, No. 2, 03.1977, p. 157-172.

Research output: Contribution to journalArticle

Conscience, Jean François ; Ruddle, Frank H. ; Skoultchi, Arthur I. ; Darlington, Gretchen J. / Somatic cell hybrids between Friend erythroleukemia cells and mouse hepatoma cells. In: Somatic Cell Genetics. 1977 ; Vol. 3, No. 2. pp. 157-172.
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