TY - JOUR
T1 - Somatic cell hybridization of mouse myeloma cells
AU - Margulies, David H.
AU - Michael Kuehl, W.
AU - Scharff, Matthew D.
N1 - Funding Information:
We thank Dr. T. V. Rajan for his close guidance in the H-Z absorptions, and Theresa Kelly, Lucille Frank, and Margaret Rubin for creative and able assistance. D. H. M. is a Medical Scientist Trainee of the NIH; W. M. K. was a Helen Hay Whitney Fellow; and M. D. S. is supported by grants from the NIH and the NSF.
PY - 1976/7
Y1 - 1976/7
N2 - Somatic cell hybrids between different mouse myeloma cell lines have been readily isolated using modifications of existing techniques. The hybrid nature of these cells was established by HAT or HAT-ouabain selective procedures, their chromosome number, and, in one case, H-2 surface antigen expression. Three hybrid cell lines are described here in detail: an IgG2b, κ{script} X IgG2a, κ{script}; an IgG1, κ{script} X IgG2b, κ{script}; and an IgG1, κ{script} X IgM, λ. In all cases, both parental types of H and L chains are expressed in the hybrid cells and no new chains are observed. However, molecules possessing disulfide-bonded mixtures of parental H and/or L chains are seen. Analysis of subclones of these hybrids indicates considerable stability in the expression of the immunoglobulins for up to 13 months. However, segregant clones no longer synthesizing one or more of the parental H or L chains arise frequently.
AB - Somatic cell hybrids between different mouse myeloma cell lines have been readily isolated using modifications of existing techniques. The hybrid nature of these cells was established by HAT or HAT-ouabain selective procedures, their chromosome number, and, in one case, H-2 surface antigen expression. Three hybrid cell lines are described here in detail: an IgG2b, κ{script} X IgG2a, κ{script}; an IgG1, κ{script} X IgG2b, κ{script}; and an IgG1, κ{script} X IgM, λ. In all cases, both parental types of H and L chains are expressed in the hybrid cells and no new chains are observed. However, molecules possessing disulfide-bonded mixtures of parental H and/or L chains are seen. Analysis of subclones of these hybrids indicates considerable stability in the expression of the immunoglobulins for up to 13 months. However, segregant clones no longer synthesizing one or more of the parental H or L chains arise frequently.
UR - http://www.scopus.com/inward/record.url?scp=0017185298&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0017185298&partnerID=8YFLogxK
U2 - 10.1016/0092-8674(76)90153-7
DO - 10.1016/0092-8674(76)90153-7
M3 - Article
C2 - 821618
AN - SCOPUS:0017185298
SN - 0092-8674
VL - 8
SP - 405
EP - 415
JO - Cell
JF - Cell
IS - 3
ER -