Somatic cell hybridization of mouse myeloma cells

David H. Margulies, W. Michael Kuehl, Matthew D. Scharff

Research output: Contribution to journalArticlepeer-review

84 Scopus citations


Somatic cell hybrids between different mouse myeloma cell lines have been readily isolated using modifications of existing techniques. The hybrid nature of these cells was established by HAT or HAT-ouabain selective procedures, their chromosome number, and, in one case, H-2 surface antigen expression. Three hybrid cell lines are described here in detail: an IgG2b, κ{script} X IgG2a, κ{script}; an IgG1, κ{script} X IgG2b, κ{script}; and an IgG1, κ{script} X IgM, λ. In all cases, both parental types of H and L chains are expressed in the hybrid cells and no new chains are observed. However, molecules possessing disulfide-bonded mixtures of parental H and/or L chains are seen. Analysis of subclones of these hybrids indicates considerable stability in the expression of the immunoglobulins for up to 13 months. However, segregant clones no longer synthesizing one or more of the parental H or L chains arise frequently.

Original languageEnglish (US)
Pages (from-to)405-415
Number of pages11
Issue number3
StatePublished - Jul 1976

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


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