Somatic cell hybridization of mouse myeloma cells

David H. Margulies, W. Michael Kuehl, Matthew D. Scharff

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

Somatic cell hybrids between different mouse myeloma cell lines have been readily isolated using modifications of existing techniques. The hybrid nature of these cells was established by HAT or HAT-ouabain selective procedures, their chromosome number, and, in one case, H-2 surface antigen expression. Three hybrid cell lines are described here in detail: an IgG2b, κ{script} X IgG2a, κ{script}; an IgG1, κ{script} X IgG2b, κ{script}; and an IgG1, κ{script} X IgM, λ. In all cases, both parental types of H and L chains are expressed in the hybrid cells and no new chains are observed. However, molecules possessing disulfide-bonded mixtures of parental H and/or L chains are seen. Analysis of subclones of these hybrids indicates considerable stability in the expression of the immunoglobulins for up to 13 months. However, segregant clones no longer synthesizing one or more of the parental H or L chains arise frequently.

Original languageEnglish (US)
Pages (from-to)405-415
Number of pages11
JournalCell
Volume8
Issue number3
DOIs
StatePublished - 1976

Fingerprint

Hybrid Cells
Immunoglobulin G
Cells
Ouabain
Surface Antigens
Chromosomes
Disulfides
Immunoglobulin M
Immunoglobulins
H-2 Antigens
Cell Line
Molecules
Clone Cells

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Somatic cell hybridization of mouse myeloma cells. / Margulies, David H.; Michael Kuehl, W.; Scharff, Matthew D.

In: Cell, Vol. 8, No. 3, 1976, p. 405-415.

Research output: Contribution to journalArticle

Margulies, David H. ; Michael Kuehl, W. ; Scharff, Matthew D. / Somatic cell hybridization of mouse myeloma cells. In: Cell. 1976 ; Vol. 8, No. 3. pp. 405-415.
@article{9e4053ac5c574578838565ea9487e977,
title = "Somatic cell hybridization of mouse myeloma cells",
abstract = "Somatic cell hybrids between different mouse myeloma cell lines have been readily isolated using modifications of existing techniques. The hybrid nature of these cells was established by HAT or HAT-ouabain selective procedures, their chromosome number, and, in one case, H-2 surface antigen expression. Three hybrid cell lines are described here in detail: an IgG2b, κ{script} X IgG2a, κ{script}; an IgG1, κ{script} X IgG2b, κ{script}; and an IgG1, κ{script} X IgM, λ. In all cases, both parental types of H and L chains are expressed in the hybrid cells and no new chains are observed. However, molecules possessing disulfide-bonded mixtures of parental H and/or L chains are seen. Analysis of subclones of these hybrids indicates considerable stability in the expression of the immunoglobulins for up to 13 months. However, segregant clones no longer synthesizing one or more of the parental H or L chains arise frequently.",
author = "Margulies, {David H.} and {Michael Kuehl}, W. and Scharff, {Matthew D.}",
year = "1976",
doi = "10.1016/0092-8674(76)90153-7",
language = "English (US)",
volume = "8",
pages = "405--415",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "3",

}

TY - JOUR

T1 - Somatic cell hybridization of mouse myeloma cells

AU - Margulies, David H.

AU - Michael Kuehl, W.

AU - Scharff, Matthew D.

PY - 1976

Y1 - 1976

N2 - Somatic cell hybrids between different mouse myeloma cell lines have been readily isolated using modifications of existing techniques. The hybrid nature of these cells was established by HAT or HAT-ouabain selective procedures, their chromosome number, and, in one case, H-2 surface antigen expression. Three hybrid cell lines are described here in detail: an IgG2b, κ{script} X IgG2a, κ{script}; an IgG1, κ{script} X IgG2b, κ{script}; and an IgG1, κ{script} X IgM, λ. In all cases, both parental types of H and L chains are expressed in the hybrid cells and no new chains are observed. However, molecules possessing disulfide-bonded mixtures of parental H and/or L chains are seen. Analysis of subclones of these hybrids indicates considerable stability in the expression of the immunoglobulins for up to 13 months. However, segregant clones no longer synthesizing one or more of the parental H or L chains arise frequently.

AB - Somatic cell hybrids between different mouse myeloma cell lines have been readily isolated using modifications of existing techniques. The hybrid nature of these cells was established by HAT or HAT-ouabain selective procedures, their chromosome number, and, in one case, H-2 surface antigen expression. Three hybrid cell lines are described here in detail: an IgG2b, κ{script} X IgG2a, κ{script}; an IgG1, κ{script} X IgG2b, κ{script}; and an IgG1, κ{script} X IgM, λ. In all cases, both parental types of H and L chains are expressed in the hybrid cells and no new chains are observed. However, molecules possessing disulfide-bonded mixtures of parental H and/or L chains are seen. Analysis of subclones of these hybrids indicates considerable stability in the expression of the immunoglobulins for up to 13 months. However, segregant clones no longer synthesizing one or more of the parental H or L chains arise frequently.

UR - http://www.scopus.com/inward/record.url?scp=0017185298&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017185298&partnerID=8YFLogxK

U2 - 10.1016/0092-8674(76)90153-7

DO - 10.1016/0092-8674(76)90153-7

M3 - Article

VL - 8

SP - 405

EP - 415

JO - Cell

JF - Cell

SN - 0092-8674

IS - 3

ER -