Soluble N-ethylmaleimide-sensitive fusion attachment proteins (SNAPs) bind to a multi-SNAP receptor complex in Golgi membranes

S. W. Whiteheart, M. Brunner, D. W. Wilson, M. Wiedmann, J. E. Rothman

Research output: Contribution to journalArticle

67 Scopus citations

Abstract

Soluble N-ethylmaleimide-sensitive fusion attachment proteins (SNAPs) are required for the binding of N-ethylmaleimide-sensitive fusion protein (NSF) to Golgi membranes and are, therefore, required for intra-Golgi transport. We report the existence of distinct α/β-SNAP and γ-SNAP-binding sites in Golgi membranes that appear to be part of the same receptor complex. Cross- linking studies with α-SNAP demonstrate that an integral membrane protein of between 30-40 kDa is the α-SNAP binding component of the multi-SNAP receptor complex. These data suggest that SNAPs function by independently binding to a multi-SNAP membrane-receptor complex, thereby activating them to serve as adaptors for the targeting of NSF.

Original languageEnglish (US)
Pages (from-to)12239-12243
Number of pages5
JournalJournal of Biological Chemistry
Volume267
Issue number17
StatePublished - 1992
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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