Soluble N-ethylmaleimide-sensitive fusion attachment proteins (SNAPs) bind to a multi-SNAP receptor complex in Golgi membranes

Sidney W. Whiteheart, Michael Brunner, Duncan W. Wilson, Martin Wiedmann, James E. Rothman

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Soluble N-ethylmaleimide-sensitive fusion attachment proteins (SNAPs) are required for the binding of N-ethylmaleimide-sensitive fusion protein (NSF) to Golgi membranes and are, therefore, required for intra-Golgi transport. We report the existence of distinct α/β-SNAP and α-SNAP-binding sites in Golgi membranes that appear to be part of the same receptor complex. Cross-linking studies with α-SNAP demonstrate that an integral membrane protein of between 30-40 kDa is the α-SNAP binding component of the multi-SNAP receptor complex. These data suggest that SNAPs function by independently binding to a multiSNAP membrane-receptor complex, thereby activating them to serve as adaptors for the targeting of NSF.

Original languageEnglish (US)
Pages (from-to)12239-12243
Number of pages5
JournalJournal of Biological Chemistry
Volume267
Issue number17
StatePublished - Jun 15 1992
Externally publishedYes

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Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
N-Ethylmaleimide-Sensitive Proteins
Ethylmaleimide
Golgi Apparatus
Fusion reactions
Membranes
Proteins
Protein Binding
Membrane Proteins
Binding Sites

ASJC Scopus subject areas

  • Biochemistry

Cite this

Soluble N-ethylmaleimide-sensitive fusion attachment proteins (SNAPs) bind to a multi-SNAP receptor complex in Golgi membranes. / Whiteheart, Sidney W.; Brunner, Michael; Wilson, Duncan W.; Wiedmann, Martin; Rothman, James E.

In: Journal of Biological Chemistry, Vol. 267, No. 17, 15.06.1992, p. 12239-12243.

Research output: Contribution to journalArticle

Whiteheart, Sidney W. ; Brunner, Michael ; Wilson, Duncan W. ; Wiedmann, Martin ; Rothman, James E. / Soluble N-ethylmaleimide-sensitive fusion attachment proteins (SNAPs) bind to a multi-SNAP receptor complex in Golgi membranes. In: Journal of Biological Chemistry. 1992 ; Vol. 267, No. 17. pp. 12239-12243.
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