TY - JOUR
T1 - Small Subunit rRNA Sequence of Enterocytozoon bieneusi and Its Potential Diagnostic Role with Use of the Polymerase Chain Reaction
AU - Weiss, Louis M.
AU - Zhu, Xiaolong
AU - Wittner, Murray
AU - Tanowitz, Herbert B.
AU - Kotler, Donald
AU - Cali, Ann
AU - Weiss, Louis M.
AU - Zhu, Xiaolong
AU - Wittner, Murray
AU - Tanowitz, Herbert B.
AU - Kotler, Donald
AU - Cali, Ann
PY - 1993/12
Y1 - 1993/12
N2 - In the past several years, microsporidia have become recognized as another important group of opportunistic infections of immunocompromised patients, especially those with AIDS. Enteric infections with the noncultivatable microsporidian parasite Enterocytozoon bieneusi have been diagnosed from AIDS patients with chronic diarrhea, malabsorption, and wasting. The incidence of infection and mechanism of transmission of these organisms in humans is unknown. Several recent tests for human pathogens have been developed using rRNA genes as diagnostic probes. Using the polymerase chain reaction and conserved regions of the small subunit rRNA (SSU-rRNA) gene, the SSU-rRNA gene of E. bieneusi was successfully cloned and subsequently sequenced. Amplification of E. bieneusi rRNA could be demonstrated from intestinal biopsies from HIV-1-infected patients infected with E. bieneusi but not from intestinal biopsies from noninfected patients. This cloned SSU-rRNA gene was used to develop improved probes for detection of E. bieneusi in tissue of infected patients.
AB - In the past several years, microsporidia have become recognized as another important group of opportunistic infections of immunocompromised patients, especially those with AIDS. Enteric infections with the noncultivatable microsporidian parasite Enterocytozoon bieneusi have been diagnosed from AIDS patients with chronic diarrhea, malabsorption, and wasting. The incidence of infection and mechanism of transmission of these organisms in humans is unknown. Several recent tests for human pathogens have been developed using rRNA genes as diagnostic probes. Using the polymerase chain reaction and conserved regions of the small subunit rRNA (SSU-rRNA) gene, the SSU-rRNA gene of E. bieneusi was successfully cloned and subsequently sequenced. Amplification of E. bieneusi rRNA could be demonstrated from intestinal biopsies from HIV-1-infected patients infected with E. bieneusi but not from intestinal biopsies from noninfected patients. This cloned SSU-rRNA gene was used to develop improved probes for detection of E. bieneusi in tissue of infected patients.
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U2 - 10.1093/infdis/168.6.1570
DO - 10.1093/infdis/168.6.1570
M3 - Article
C2 - 8245549
AN - SCOPUS:0027437530
SN - 0022-1899
VL - 168
SP - 1570
EP - 1575
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 6
ER -