Small bowel transplantation in children

An immunohistochemical study of intestinal grafts

G. Fromont, N. Cerf-Bensussan, N. Patey, D. Canioni, C. Rambaud, O. Goulet, Dominique M. Jan, Y. Revillon, C. Ricour, N. Brousse

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Seven children with short bowel syndrome underwent small bowel allografting. Episodes of early rejection were observed in five patients who received a graft from paediatric or adult donors but not in two patients who received a neonatal graft. This study aimed, firstly, to define immunohistochemical parameters accompanying rejection and, secondly, to compare immunohistochemical parameters in neonatal grafts with those in grafts from older donors. An immunohistochemical analysis was performed on 85 intestinal biopsy specimens taken for monitoring the transplants. Acute histological rejection was associated with pericryptic infiltrates of CD3+TcRαβ+T cells containing clusters of CD8+ cells, numerous CD25+ cells, and increased numbers of CD68+ macrophages. These changes were associated with the appearance of major histocompatibility (MHC) class II antigens on crypt enterocytes and with an appreciable increase in the expression of E-selectin on mucosal endothelial cells. Immunohistochemistry was useful in predicting rejection by showing the appearance of pericryptic CD25+ T cells 48 hours before the first histological lesions of crypt necrosis. Comparison of neonatal grafts with grafts from older donors did not show any significant difference in the density of CD68+ macrophages or in the endothelial expression of intercellular adhesion molecule-1, vascular cell adhesion molecule-1, or E-selectin. In contrast to grafts from older donors, however, neonatal grafts did not express MHC class II antigens on epithelial cells and contained very low numbers of intraepithelial lymphocytes. These data indicate, firstly, that immunohistochemistry is useful for monitoring intestinal transplants and, secondly, that the better clinical tolerance of neonatal allografts may be related to the lower immunogenicity of the neonatal epithelium.

Original languageEnglish (US)
Pages (from-to)783-790
Number of pages8
JournalGut
Volume37
Issue number6
StatePublished - 1995
Externally publishedYes

Fingerprint

Transplantation
Transplants
Tissue Donors
E-Selectin
Histocompatibility Antigens Class II
Immunohistochemistry
Macrophages
Short Bowel Syndrome
Transplantation Tolerance
T-Lymphocytes
Vascular Cell Adhesion Molecule-1
Enterocytes
Homologous Transplantation
Lymphocyte Count
Intercellular Adhesion Molecule-1
Necrosis
Epithelium
Endothelial Cells
Cell Count
Epithelial Cells

Keywords

  • Bowel allograft
  • Immunohistochemistry
  • Paediatric

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Fromont, G., Cerf-Bensussan, N., Patey, N., Canioni, D., Rambaud, C., Goulet, O., ... Brousse, N. (1995). Small bowel transplantation in children: An immunohistochemical study of intestinal grafts. Gut, 37(6), 783-790.

Small bowel transplantation in children : An immunohistochemical study of intestinal grafts. / Fromont, G.; Cerf-Bensussan, N.; Patey, N.; Canioni, D.; Rambaud, C.; Goulet, O.; Jan, Dominique M.; Revillon, Y.; Ricour, C.; Brousse, N.

In: Gut, Vol. 37, No. 6, 1995, p. 783-790.

Research output: Contribution to journalArticle

Fromont, G, Cerf-Bensussan, N, Patey, N, Canioni, D, Rambaud, C, Goulet, O, Jan, DM, Revillon, Y, Ricour, C & Brousse, N 1995, 'Small bowel transplantation in children: An immunohistochemical study of intestinal grafts', Gut, vol. 37, no. 6, pp. 783-790.
Fromont G, Cerf-Bensussan N, Patey N, Canioni D, Rambaud C, Goulet O et al. Small bowel transplantation in children: An immunohistochemical study of intestinal grafts. Gut. 1995;37(6):783-790.
Fromont, G. ; Cerf-Bensussan, N. ; Patey, N. ; Canioni, D. ; Rambaud, C. ; Goulet, O. ; Jan, Dominique M. ; Revillon, Y. ; Ricour, C. ; Brousse, N. / Small bowel transplantation in children : An immunohistochemical study of intestinal grafts. In: Gut. 1995 ; Vol. 37, No. 6. pp. 783-790.
@article{21c8d8c89954435da15591140961d78f,
title = "Small bowel transplantation in children: An immunohistochemical study of intestinal grafts",
abstract = "Seven children with short bowel syndrome underwent small bowel allografting. Episodes of early rejection were observed in five patients who received a graft from paediatric or adult donors but not in two patients who received a neonatal graft. This study aimed, firstly, to define immunohistochemical parameters accompanying rejection and, secondly, to compare immunohistochemical parameters in neonatal grafts with those in grafts from older donors. An immunohistochemical analysis was performed on 85 intestinal biopsy specimens taken for monitoring the transplants. Acute histological rejection was associated with pericryptic infiltrates of CD3+TcRαβ+T cells containing clusters of CD8+ cells, numerous CD25+ cells, and increased numbers of CD68+ macrophages. These changes were associated with the appearance of major histocompatibility (MHC) class II antigens on crypt enterocytes and with an appreciable increase in the expression of E-selectin on mucosal endothelial cells. Immunohistochemistry was useful in predicting rejection by showing the appearance of pericryptic CD25+ T cells 48 hours before the first histological lesions of crypt necrosis. Comparison of neonatal grafts with grafts from older donors did not show any significant difference in the density of CD68+ macrophages or in the endothelial expression of intercellular adhesion molecule-1, vascular cell adhesion molecule-1, or E-selectin. In contrast to grafts from older donors, however, neonatal grafts did not express MHC class II antigens on epithelial cells and contained very low numbers of intraepithelial lymphocytes. These data indicate, firstly, that immunohistochemistry is useful for monitoring intestinal transplants and, secondly, that the better clinical tolerance of neonatal allografts may be related to the lower immunogenicity of the neonatal epithelium.",
keywords = "Bowel allograft, Immunohistochemistry, Paediatric",
author = "G. Fromont and N. Cerf-Bensussan and N. Patey and D. Canioni and C. Rambaud and O. Goulet and Jan, {Dominique M.} and Y. Revillon and C. Ricour and N. Brousse",
year = "1995",
language = "English (US)",
volume = "37",
pages = "783--790",
journal = "Gut",
issn = "0017-5749",
publisher = "BMJ Publishing Group",
number = "6",

}

TY - JOUR

T1 - Small bowel transplantation in children

T2 - An immunohistochemical study of intestinal grafts

AU - Fromont, G.

AU - Cerf-Bensussan, N.

AU - Patey, N.

AU - Canioni, D.

AU - Rambaud, C.

AU - Goulet, O.

AU - Jan, Dominique M.

AU - Revillon, Y.

AU - Ricour, C.

AU - Brousse, N.

PY - 1995

Y1 - 1995

N2 - Seven children with short bowel syndrome underwent small bowel allografting. Episodes of early rejection were observed in five patients who received a graft from paediatric or adult donors but not in two patients who received a neonatal graft. This study aimed, firstly, to define immunohistochemical parameters accompanying rejection and, secondly, to compare immunohistochemical parameters in neonatal grafts with those in grafts from older donors. An immunohistochemical analysis was performed on 85 intestinal biopsy specimens taken for monitoring the transplants. Acute histological rejection was associated with pericryptic infiltrates of CD3+TcRαβ+T cells containing clusters of CD8+ cells, numerous CD25+ cells, and increased numbers of CD68+ macrophages. These changes were associated with the appearance of major histocompatibility (MHC) class II antigens on crypt enterocytes and with an appreciable increase in the expression of E-selectin on mucosal endothelial cells. Immunohistochemistry was useful in predicting rejection by showing the appearance of pericryptic CD25+ T cells 48 hours before the first histological lesions of crypt necrosis. Comparison of neonatal grafts with grafts from older donors did not show any significant difference in the density of CD68+ macrophages or in the endothelial expression of intercellular adhesion molecule-1, vascular cell adhesion molecule-1, or E-selectin. In contrast to grafts from older donors, however, neonatal grafts did not express MHC class II antigens on epithelial cells and contained very low numbers of intraepithelial lymphocytes. These data indicate, firstly, that immunohistochemistry is useful for monitoring intestinal transplants and, secondly, that the better clinical tolerance of neonatal allografts may be related to the lower immunogenicity of the neonatal epithelium.

AB - Seven children with short bowel syndrome underwent small bowel allografting. Episodes of early rejection were observed in five patients who received a graft from paediatric or adult donors but not in two patients who received a neonatal graft. This study aimed, firstly, to define immunohistochemical parameters accompanying rejection and, secondly, to compare immunohistochemical parameters in neonatal grafts with those in grafts from older donors. An immunohistochemical analysis was performed on 85 intestinal biopsy specimens taken for monitoring the transplants. Acute histological rejection was associated with pericryptic infiltrates of CD3+TcRαβ+T cells containing clusters of CD8+ cells, numerous CD25+ cells, and increased numbers of CD68+ macrophages. These changes were associated with the appearance of major histocompatibility (MHC) class II antigens on crypt enterocytes and with an appreciable increase in the expression of E-selectin on mucosal endothelial cells. Immunohistochemistry was useful in predicting rejection by showing the appearance of pericryptic CD25+ T cells 48 hours before the first histological lesions of crypt necrosis. Comparison of neonatal grafts with grafts from older donors did not show any significant difference in the density of CD68+ macrophages or in the endothelial expression of intercellular adhesion molecule-1, vascular cell adhesion molecule-1, or E-selectin. In contrast to grafts from older donors, however, neonatal grafts did not express MHC class II antigens on epithelial cells and contained very low numbers of intraepithelial lymphocytes. These data indicate, firstly, that immunohistochemistry is useful for monitoring intestinal transplants and, secondly, that the better clinical tolerance of neonatal allografts may be related to the lower immunogenicity of the neonatal epithelium.

KW - Bowel allograft

KW - Immunohistochemistry

KW - Paediatric

UR - http://www.scopus.com/inward/record.url?scp=0028884732&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028884732&partnerID=8YFLogxK

M3 - Article

VL - 37

SP - 783

EP - 790

JO - Gut

JF - Gut

SN - 0017-5749

IS - 6

ER -