Single-Cell and Single-Molecule Analysis of Gene Expression Regulation

Maria Vera Ugalde, Jeetayu Biswas, Adrien Senecal, Robert H. Singer, Hye Yoon Park

Research output: Contribution to journalReview article

43 Scopus citations


Recent advancements in single-cell and single-molecule imaging technologies have resolved biological processes in time and space that are fundamental to understanding the regulation of gene expression. Observations of single-molecule events in their cellular context have revealed highly dynamic aspects of transcriptional and post-transcriptional control in eukaryotic cells. This approach can relate transcription with mRNA abundance and lifetimes. Another key aspect of single-cell analysis is the cell-to-cell variability among populations of cells. Definition of heterogeneity has revealed stochastic processes, determined characteristics of under-represented cell types or transitional states, and integrated cellular behaviors in the context of multicellular organisms. In this review, we discuss novel aspects of gene expression of eukaryotic cells and multicellular organisms revealed by the latest advances in single-cell and single-molecule imaging technology.

Original languageEnglish (US)
Pages (from-to)267-291
Number of pages25
JournalAnnual Review of Genetics
StatePublished - Nov 23 2016


  • Gene expression
  • mRNA localization
  • Single cell
  • Single-molecule imaging
  • Transcription
  • Translation

ASJC Scopus subject areas

  • Genetics

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