Silymarin downregulates COX-2 expression and attenuates hyperlipidemia during NDEA-induced rat hepatocellular carcinoma

Gopalakrishnan Ramakrishnan, Carmen Martha Elinos-Báez, Sundaram Jagan, Titto A. Augustine, Sattu Kamaraj, Pandi Anandakumar, Thiruvengadam Devaki

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Silymarin is a naturally available bioflavonoid and is a strong antioxidant with a capacity to inhibit the formation of tumors in several cancer models. In the present study, we investigated whether dietary supplementation of silymarin has any role in lipid components, lipid-metabolizing enzymes, free fatty acid profile, and expression of cyclooxygenase-2 (COX-2) in N-nitrosodiethylamine (NDEA)-induced hepatocellular carcinoma in rats. NDEA-induced rats showed severe hyperlipidemia along with upregulated expression of COX-2 as revealed by western blotting and immunohistochemistry. Dietary silymarin supplementation attenuated this hyperlipidemia and downregulated the expression of COX-2. Thus we conclude that compounds like silymarin with potent hypolipidemic effect are strong candidates as chemopreventive agents for the treatment of liver cancer.

Original languageEnglish (US)
Pages (from-to)53-61
Number of pages9
JournalMolecular and Cellular Biochemistry
Volume313
Issue number1-2
DOIs
StatePublished - Jun 2008
Externally publishedYes

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Silymarin
Diethylnitrosamine
Cyclooxygenase 2
Hyperlipidemias
Rats
Hepatocellular Carcinoma
Down-Regulation
Dietary Supplements
Lipids
Liver Neoplasms
Nonesterified Fatty Acids
Flavonoids
Liver
Tumors
Neoplasms
Antioxidants
Western Blotting
Immunohistochemistry
Enzymes

Keywords

  • COX-2
  • Hepatocellular carcinoma
  • HMG-CoAreductase
  • Hyperlipidemia
  • N-nitrosodiethylamine
  • Silymarin

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Silymarin downregulates COX-2 expression and attenuates hyperlipidemia during NDEA-induced rat hepatocellular carcinoma. / Ramakrishnan, Gopalakrishnan; Elinos-Báez, Carmen Martha; Jagan, Sundaram; Augustine, Titto A.; Kamaraj, Sattu; Anandakumar, Pandi; Devaki, Thiruvengadam.

In: Molecular and Cellular Biochemistry, Vol. 313, No. 1-2, 06.2008, p. 53-61.

Research output: Contribution to journalArticle

Ramakrishnan, Gopalakrishnan ; Elinos-Báez, Carmen Martha ; Jagan, Sundaram ; Augustine, Titto A. ; Kamaraj, Sattu ; Anandakumar, Pandi ; Devaki, Thiruvengadam. / Silymarin downregulates COX-2 expression and attenuates hyperlipidemia during NDEA-induced rat hepatocellular carcinoma. In: Molecular and Cellular Biochemistry. 2008 ; Vol. 313, No. 1-2. pp. 53-61.
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