Signal transduction by platelet adenylate cyclase: Alterations in depressed patients may reflect impairment in the coordinated integration of cellular signals (coincidence detection)

John J. Mooney, Jacqueline A. Samson, Nancy L. McHale, Robin Colodzin, Jonathan E. Alpert, Martha Koutsos, Joseph J. Schildkraut

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Background: Adenylate cyclase (AC) responds to distinct but coincident signals from the agonist-stimulated G-protein G(s) and the inhibitory G- protein G(i) by generating a greater output signal-to-noise ratio-i.e., agonist-stimulated to basal ratio (fold-stimulation)-through coincidence detection than that generated by a single input (G(s)) alone. Such coincidence detection by murine brain AC was found to be enhanced during chronic antidepressant treatment with imipramine. Methods: We examined and compared the basal, agonist-stimulated, and guanosine 5'-3-O- (thio)triphosphate (GTPγS) or AlF4 ion postreceptor-stimulated AC activities in mononuclear leukocytes and platelets from the same blood specimens obtained from depressed patients (n = 27) and control subjects (n = 19). Results: In all subjects, the differences (ΔGTPγS or ΔAlF4) between postreceptor measures of AC in mononuclear leukocytes (where AC is regulated by G(s) but not by G(i)) and platelets (where AC is regulated by both G(s) and G(i)) were highly significant. In controls, the relationships between ΔGTPγS or ΔAlF4 and basal, agonist-stimulated, and the fold-stimulation of agonist-stimulated platelet AC resembled the regulation of AC by G(i) in model membrane systems. Comparable relationships between ΔGTPγS or ΔAlF4 and basal, agonist-stimulated, and the fold-stimulation of agonist-stimulated platelet AC activities were not observed in depressed patients. Conclusions: Our results suggest that in controls, platelet AC enzyme activity is determined (in part) by the coordinated integration of signals from G(s) and G(i) through coincidence detection, while such coincidence detection by platelet AC may be impaired in patients with depressive disorders.

Original languageEnglish (US)
Pages (from-to)574-583
Number of pages10
JournalBiological Psychiatry
Volume43
Issue number8
DOIs
StatePublished - Apr 15 1998
Externally publishedYes

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Adenylyl Cyclases
Signal Transduction
Blood Platelets
Mononuclear Leukocytes
GTP-Binding Proteins
Guanosine 5'-O-(3-Thiotriphosphate)
Imipramine
Signal-To-Noise Ratio
Depressive Disorder
Antidepressive Agents
Membranes
tetrafluoroaluminate
Brain

Keywords

  • Adenylate cyclase
  • Blood cells
  • Depression
  • Signal transduction

ASJC Scopus subject areas

  • Biological Psychiatry

Cite this

Signal transduction by platelet adenylate cyclase : Alterations in depressed patients may reflect impairment in the coordinated integration of cellular signals (coincidence detection). / Mooney, John J.; Samson, Jacqueline A.; McHale, Nancy L.; Colodzin, Robin; Alpert, Jonathan E.; Koutsos, Martha; Schildkraut, Joseph J.

In: Biological Psychiatry, Vol. 43, No. 8, 15.04.1998, p. 574-583.

Research output: Contribution to journalArticle

Mooney, John J. ; Samson, Jacqueline A. ; McHale, Nancy L. ; Colodzin, Robin ; Alpert, Jonathan E. ; Koutsos, Martha ; Schildkraut, Joseph J. / Signal transduction by platelet adenylate cyclase : Alterations in depressed patients may reflect impairment in the coordinated integration of cellular signals (coincidence detection). In: Biological Psychiatry. 1998 ; Vol. 43, No. 8. pp. 574-583.
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T2 - Alterations in depressed patients may reflect impairment in the coordinated integration of cellular signals (coincidence detection)

AU - Mooney, John J.

AU - Samson, Jacqueline A.

AU - McHale, Nancy L.

AU - Colodzin, Robin

AU - Alpert, Jonathan E.

AU - Koutsos, Martha

AU - Schildkraut, Joseph J.

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AB - Background: Adenylate cyclase (AC) responds to distinct but coincident signals from the agonist-stimulated G-protein G(s) and the inhibitory G- protein G(i) by generating a greater output signal-to-noise ratio-i.e., agonist-stimulated to basal ratio (fold-stimulation)-through coincidence detection than that generated by a single input (G(s)) alone. Such coincidence detection by murine brain AC was found to be enhanced during chronic antidepressant treatment with imipramine. Methods: We examined and compared the basal, agonist-stimulated, and guanosine 5'-3-O- (thio)triphosphate (GTPγS) or AlF4 ion postreceptor-stimulated AC activities in mononuclear leukocytes and platelets from the same blood specimens obtained from depressed patients (n = 27) and control subjects (n = 19). Results: In all subjects, the differences (ΔGTPγS or ΔAlF4) between postreceptor measures of AC in mononuclear leukocytes (where AC is regulated by G(s) but not by G(i)) and platelets (where AC is regulated by both G(s) and G(i)) were highly significant. In controls, the relationships between ΔGTPγS or ΔAlF4 and basal, agonist-stimulated, and the fold-stimulation of agonist-stimulated platelet AC resembled the regulation of AC by G(i) in model membrane systems. Comparable relationships between ΔGTPγS or ΔAlF4 and basal, agonist-stimulated, and the fold-stimulation of agonist-stimulated platelet AC activities were not observed in depressed patients. Conclusions: Our results suggest that in controls, platelet AC enzyme activity is determined (in part) by the coordinated integration of signals from G(s) and G(i) through coincidence detection, while such coincidence detection by platelet AC may be impaired in patients with depressive disorders.

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