Signal requirements for lymphocyte activation: Role of a T-cell growth factor produced by guinea pig peritoneal exudate lymphocytes

Anita Corman Weinblatt, Joost J. Oppenheim, David L. Rosenstreich

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Peritoneal exudate lymphocytes (PEL) from immunized guinea pigs, when pulsed with antigen, rapidly release a T-cell stimulatory factor (TSF). TSF nonspecifically enhances the proliferation of purified guinea pig T cells in the presence of another signal such as PMA, PHA, or Con A. On a per cell basis, antigen-pulsed PEL produce about 14 times more activity than similarly stimulated lymph node lymphocytes. Several lines of evidence support the view that TSF is the guinea pig equivalent of TCGF (IL-2). TSF containing supernatants have IL-2 activity when assayed on the IL-2-dependent CT6 cell line. When TSF containing supernatants were absorbed with CT6 cells, there was a significant decrease of both TSF activity as assessed on guinea pig T cells as well as IL-2 activity as assessed by the CT6 assay. Additionally, partially purified human and mouse IL-2 have TSF activity, while the macrophage product, IL-1, has no TSF activity. After chromatography on a S-200 column, TSF activity and IL-2 activity coelute at an apparent molecular weight of 19,000.

Original languageEnglish (US)
Pages (from-to)332-342
Number of pages11
JournalCellular Immunology
Volume68
Issue number2
DOIs
StatePublished - 1982
Externally publishedYes

Fingerprint

TCF Transcription Factors
Exudates and Transudates
Lymphocyte Activation
Interleukin-2
Guinea Pigs
Lymphocytes
T-Lymphocytes
Antigens
Interleukin-1
Chromatography
Molecular Weight
Lymph Nodes
Macrophages
Cell Line

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Signal requirements for lymphocyte activation : Role of a T-cell growth factor produced by guinea pig peritoneal exudate lymphocytes. / Weinblatt, Anita Corman; Oppenheim, Joost J.; Rosenstreich, David L.

In: Cellular Immunology, Vol. 68, No. 2, 1982, p. 332-342.

Research output: Contribution to journalArticle

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