Sidekick-1 is upregulated in glomeruli in HIV-associated nephropathy

Lewis Kaufman, Kayo Hayashi, Michael J. Ross, Michael D. Ross, Paul E. Klotman

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Infection of podocytes by HIV-1 induces unique changes in phenotype, which contribute to the pathogenesis of glomerular disease in HIV-associated nephropathy (HIVAN). The host genetic pathways altered by HIV-1 infection that are responsible for these phenotypic changes are largely unknown. For identifying such pathways, representational difference analysis was performed comparing cDNA from HIV-1 transgenic podocytes with nontransgenic controls. In this way, a gene named sidekick-1 (sdk-1) was cloned, a transmembrane protein of the Ig superfamily that is highly upregulated in HIV-1 transgenic podocytes. Sdk-1 and its ortholog, sidekick-2 (sdk-2), were recently shown to guide axonal terminals to specific synapses in developing neurons. Their presence and role in other organs, including the kidney, has not been described. The current study demonstrates developmental expression of both sdk-1 and sdk-2 and a tight spatial and temporal regulation of these genes in kidney. During nephrogenesis, sidekick expression was observed first in ureteric bud and ureteric bud-derived tissues in a pattern similar to other genes known to play important roles in branching morphogenesis. In adult murine renal tissue, sidekick proteins were seen in glomeruli at low levels, and expression of sdk-1 was greatly upregulated in diseased HIV-1 transgenic mouse kidneys. In a human HIVAN kidney biopsy, sidekick expression was increased in glomeruli in a pattern consistent with the mouse model. It is proposed that the dysregulation of sdk-1 protein may play an important role in HIVAN pathogenesis.

Original languageEnglish (US)
Pages (from-to)1721-1730
Number of pages10
JournalJournal of the American Society of Nephrology
Volume15
Issue number7
DOIs
StatePublished - Jul 2004
Externally publishedYes

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AIDS-Associated Nephropathy
HIV-1
Podocytes
Kidney
Genes
Proteins
Morphogenesis
Synapses
Transgenic Mice
HIV Infections
Complementary DNA
Phenotype
Biopsy
Neurons
Infection

ASJC Scopus subject areas

  • Nephrology

Cite this

Sidekick-1 is upregulated in glomeruli in HIV-associated nephropathy. / Kaufman, Lewis; Hayashi, Kayo; Ross, Michael J.; Ross, Michael D.; Klotman, Paul E.

In: Journal of the American Society of Nephrology, Vol. 15, No. 7, 07.2004, p. 1721-1730.

Research output: Contribution to journalArticle

Kaufman, Lewis ; Hayashi, Kayo ; Ross, Michael J. ; Ross, Michael D. ; Klotman, Paul E. / Sidekick-1 is upregulated in glomeruli in HIV-associated nephropathy. In: Journal of the American Society of Nephrology. 2004 ; Vol. 15, No. 7. pp. 1721-1730.
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