Serologic evidence for reactivation of cryptococcosis in solid-organ transplant recipients

D. C. Saha, David L. Goldman, X. Shao, A. Casadevall, S. Husain, A. P. Limaye, M. Lyon, J. Somani, K. Pursell, T. L. Pruett, N. Singh

Research output: Contribution to journalArticle

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Abstract

Cryptococcosis is a significant infection with a high mortality in solid-organ transplant recipients. Nonetheless, the pathogenesis of this disease is poorly understood. It has been hypothesized that cryptococcosis may result from either primary infection or reactivation of a latent infection. Sera were obtained from transplant recipients prior to transplantation and at the time they developed cryptococcosis. Control sera were obtained before and after transplant from patients who did not develop cryptococcosis. Sera were tested for antibodies against Cryptococcus neoformans by using an immunoblot assay. Antibody responses were also compared with those observed in sera from rats with experimental pulmonary cryptococcosis. In all, 52% of the transplant recipients who developed cryptococcosis exhibited serologic evidence of cryptococcal infection before transplantation. These patients developed cryptococcosis significantly earlier after transplant than patients without preexisting reactivity did (5.6 ± 3.4 months compared to 40.6 ± 63.8 months, respectively [P = 0.0011]). The results from our study suggest that a substantial proportion of transplant-associated cryptococcosis cases result from the reactivation of a latent infection. These findings also highlight the potential utility of serologic studies in identifying patients at risk for the development of cryptococcosis after transplantation.

Original languageEnglish (US)
Pages (from-to)1550-1554
Number of pages5
JournalClinical and Vaccine Immunology
Volume14
Issue number12
DOIs
StatePublished - Dec 2007

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Cryptococcosis
Transplants
Transplantation (surgical)
Infection
Transplantation
Serum
Antibodies
Transplant Recipients
Rats
Assays
Cryptococcus neoformans
Antibody Formation
Lung
Mortality

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Immunology
  • Immunology and Allergy
  • Microbiology (medical)

Cite this

Serologic evidence for reactivation of cryptococcosis in solid-organ transplant recipients. / Saha, D. C.; Goldman, David L.; Shao, X.; Casadevall, A.; Husain, S.; Limaye, A. P.; Lyon, M.; Somani, J.; Pursell, K.; Pruett, T. L.; Singh, N.

In: Clinical and Vaccine Immunology, Vol. 14, No. 12, 12.2007, p. 1550-1554.

Research output: Contribution to journalArticle

Saha, DC, Goldman, DL, Shao, X, Casadevall, A, Husain, S, Limaye, AP, Lyon, M, Somani, J, Pursell, K, Pruett, TL & Singh, N 2007, 'Serologic evidence for reactivation of cryptococcosis in solid-organ transplant recipients', Clinical and Vaccine Immunology, vol. 14, no. 12, pp. 1550-1554. https://doi.org/10.1128/CVI.00242-07
Saha, D. C. ; Goldman, David L. ; Shao, X. ; Casadevall, A. ; Husain, S. ; Limaye, A. P. ; Lyon, M. ; Somani, J. ; Pursell, K. ; Pruett, T. L. ; Singh, N. / Serologic evidence for reactivation of cryptococcosis in solid-organ transplant recipients. In: Clinical and Vaccine Immunology. 2007 ; Vol. 14, No. 12. pp. 1550-1554.
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