Serologic Diagnosis of Lyme Disease using Recombinant Outer Surface Proteins A and B and Flagellin

Erol Fikrig, Edward D. Huguenel, Robert Berland, Daniel W. Rahn, John A. Hardin, Richard A. Flavell

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

Recombinant (r) outer surface proteins (Osp) A and B, flagellin, and an immunogenic region of flagellin (41-G) from Borrelia burgdorferi were evaluated using immunoblot and ELISA for usefulness as substrates in diagnostic testing for Lyme disease. Antibodies to rOspA, rOspB, and r-flagellin were detected by immunoblot and ELISA using the recombinant proteins. Patients with late disease responded to rOspA, rOspB, and r-flagellin, or only to r-flagellin, whereas patients with early disease showed no response or responded only to r-flagellin. Patients who developed antibodies to r-flagellin also responded to 41-G. The data suggest that recombinant B. burgdorferi antigens can serve as substrates for immunoblot and ELISA, which may be helpful in diagnosing Lyme disease.

Original languageEnglish (US)
Pages (from-to)1127-1132
Number of pages6
JournalJournal of Infectious Diseases
Volume165
Issue number6
DOIs
StatePublished - Jun 1992

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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    Fikrig, E., Huguenel, E. D., Berland, R., Rahn, D. W., Hardin, J. A., & Flavell, R. A. (1992). Serologic Diagnosis of Lyme Disease using Recombinant Outer Surface Proteins A and B and Flagellin. Journal of Infectious Diseases, 165(6), 1127-1132. https://doi.org/10.1093/infdis/165.6.1127