Sequestration of Mycobacterium tuberculosis in tight vacuoles in vivo in lung macrophages of mice infected by the respiratory route

A. L. Moreira, J. Wang, L. Tsenova-Berkova, W. Hellmann, V. H. Freedman, G. Kaplan

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40 Scopus citations


Following aerosol infection of mice with Mycobacterium tuberculosis, single mycobacteria or pairs of bacilli were observed within individual phagocytic vacuoles hound by tightly apposed vacuolar membranes. The virulent organism was not observed free in the cytoplasm of the parasitized cells or in the extracellular space of the lung granulomata. This study indicates that in vivo, virulent mycobacteria survive and probably replicate within a unique tight vacuole in the infected phagocyte within the lung.

Original languageEnglish (US)
Pages (from-to)305-308
Number of pages4
JournalInfection and Immunity
Issue number1
Publication statusPublished - Jan 11 1997
Externally publishedYes


ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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