TY - JOUR
T1 - Sequence of a human MAP‐2 region sharing epitopes with Alzheimer neurofibrillary tangles
AU - Dammerman, M.
AU - Yen, S. ‐H
AU - Shafit‐Zagardo, B.
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1989/12
Y1 - 1989/12
N2 - Microtubule‐associated protein 2 (MAP‐2), an abundant neuronal protein, consists of a short microtubule‐binding domain and a long projection arm. MAP‐2 shares epitopes with Alzheimer neurofibrillary tangles (NFT). However, most anti‐MAP‐2 antibodies do not stain detergent‐extracted NFT, and the role of MAP‐2 in NFT formation has therefore been unclear. We have determined the sequence of a 1.7 kb partial MAP‐2 cDNA encoding at least three NFT epitopes. The epitopes are not removed by detergent extraction of tangle preparations, suggesting that they are integral components of NFT. Expression vectors containing restriction fragments of the cDNA were used to assign the epitopes to a 51‐amino‐acid region near the end of the MAP‐2 projection arm, distal to the microtubule.
AB - Microtubule‐associated protein 2 (MAP‐2), an abundant neuronal protein, consists of a short microtubule‐binding domain and a long projection arm. MAP‐2 shares epitopes with Alzheimer neurofibrillary tangles (NFT). However, most anti‐MAP‐2 antibodies do not stain detergent‐extracted NFT, and the role of MAP‐2 in NFT formation has therefore been unclear. We have determined the sequence of a 1.7 kb partial MAP‐2 cDNA encoding at least three NFT epitopes. The epitopes are not removed by detergent extraction of tangle preparations, suggesting that they are integral components of NFT. Expression vectors containing restriction fragments of the cDNA were used to assign the epitopes to a 51‐amino‐acid region near the end of the MAP‐2 projection arm, distal to the microtubule.
KW - Alzheimer's disease
KW - microtubule‐associated protein 2
KW - paired helical filaments
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U2 - 10.1002/jnr.490240405
DO - 10.1002/jnr.490240405
M3 - Article
C2 - 2481044
AN - SCOPUS:0024801318
VL - 24
SP - 487
EP - 495
JO - Journal of Neuroscience Research
JF - Journal of Neuroscience Research
SN - 0360-4012
IS - 4
ER -