Sequence of a human MAP‐2 region sharing epitopes with Alzheimer neurofibrillary tangles

M. Dammerman, S. ‐H Yen, B. Shafit‐Zagardo

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Microtubule‐associated protein 2 (MAP‐2), an abundant neuronal protein, consists of a short microtubule‐binding domain and a long projection arm. MAP‐2 shares epitopes with Alzheimer neurofibrillary tangles (NFT). However, most anti‐MAP‐2 antibodies do not stain detergent‐extracted NFT, and the role of MAP‐2 in NFT formation has therefore been unclear. We have determined the sequence of a 1.7 kb partial MAP‐2 cDNA encoding at least three NFT epitopes. The epitopes are not removed by detergent extraction of tangle preparations, suggesting that they are integral components of NFT. Expression vectors containing restriction fragments of the cDNA were used to assign the epitopes to a 51‐amino‐acid region near the end of the MAP‐2 projection arm, distal to the microtubule.

Original languageEnglish (US)
Pages (from-to)487-495
Number of pages9
JournalJournal of Neuroscience Research
Volume24
Issue number4
DOIs
StatePublished - Dec 1989

Keywords

  • Alzheimer's disease
  • microtubule‐associated protein 2
  • paired helical filaments

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

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