Separation of temporal control and trans-acting modulation of flagellin and chemotaxis genes in Caulobacter

Ruth Bryan, Robert Champer, Suely Gomes, Bert Ely, Lucille Shapiro

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The genes involved in the biogenesis of the flagellum and the chemotaxis machinery are temporally regulated during the Caulobacter crescentus cell cycle. Using plasmid complementation, we have mapped the extent of the flaY and flaE genes. These genes function in trans to regulate the expression of the flagellin genes and the chemotaxis genes. We have found that the trans regulation that modulates the amounts of the flagellins and the chemotaxis proteins can be separated from the temporal control of fla and che gene expression. This conclusion is based on two observations: (1) the low level of synthesis of flagellins and chemotaxis proteins in flaY and flaE mutant strains occurred at the correct time in the cell cycle, and (2) complementation with plasmids containing intact flaY and flaE genes resulted in the synthesis of normal levels of flagellins and chemotaxis gene products with the maintenance of temporal cell cycle control.

Original languageEnglish (US)
Pages (from-to)300-306
Number of pages7
JournalMGG Molecular & General Genetics
Volume206
Issue number2
DOIs
StatePublished - Feb 1987

Fingerprint

Caulobacter
Flagellin
Chemotaxis
Genes
Cell Cycle
Plasmids
Caulobacter crescentus
Gene Expression
Flagella
Cell Cycle Checkpoints
Proteins
Maintenance

Keywords

  • Caulobacter
  • Chemotaxis proteins
  • Flagellins, Temporal control
  • Trans-acting regulation

ASJC Scopus subject areas

  • Genetics

Cite this

Separation of temporal control and trans-acting modulation of flagellin and chemotaxis genes in Caulobacter. / Bryan, Ruth; Champer, Robert; Gomes, Suely; Ely, Bert; Shapiro, Lucille.

In: MGG Molecular & General Genetics, Vol. 206, No. 2, 02.1987, p. 300-306.

Research output: Contribution to journalArticle

Bryan, Ruth ; Champer, Robert ; Gomes, Suely ; Ely, Bert ; Shapiro, Lucille. / Separation of temporal control and trans-acting modulation of flagellin and chemotaxis genes in Caulobacter. In: MGG Molecular & General Genetics. 1987 ; Vol. 206, No. 2. pp. 300-306.
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