Separating kindling and LTP: Lessons from studies of PKM zeta in developing and adult rats

James G. Heida, Dario J. Englot, Todd C. Sacktor, Hal Blumenfeld, Solomon L. Moshé

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

The kindling model of temporal lobe epilepsy (TLE) and the memory model of long-term potentiation (LTP) may have common underlying mechanisms. This is evident by the demonstration that certain signaling molecules play a key role in both. Recently, a brain specific isoform of protein kinase C (PKMζ) has been shown to play a significant role in both maintaining LTP and memory storage. We were interested in determining if this kinase had a crossover role in kindling-induced epileptogenesis. Using developing and adult rats we examined the role of PKMζ in kindling. In developing (P15) rats we determined the effect of PKMζ on retention of amygdala kindling and kindling rate by intra-amygdala administration of a selective PKMζ antagonist, ZIP (10 nmol). In adult rats we examined the effect of PKMζ inhibition, ZIP (10 nmol), on after discharge (AD) thresholds and kindling retention using rapid hippocampal kindling. Inhibition of PKMζ by the antagonist ZIP did not affect kindling rate or retention in developing rats. In addition there was also no observed effect on AD thresholds and kindling retention in adult rats. Our results show that, despite the similarities between kindling and LTP in their induction, there is dissociation in the role that PKMζ plays within the two in maintenance. This may be of importance in establishing a separation between the pathophysiological processes involved in sustaining kindling and the physiological mechanisms involved in maintaining LTP and memory storage.

Original languageEnglish (US)
Pages (from-to)229-232
Number of pages4
JournalNeuroscience Letters
Volume453
Issue number3
DOIs
StatePublished - Apr 10 2009

Keywords

  • Development
  • Protein kinase
  • Rat
  • Seizures

ASJC Scopus subject areas

  • Neuroscience(all)

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