Sensitive and specific detection of toxoplasma DNA in an experimental murine model: Use of Toxoplasma gondii-specific cDNA and the polymerase chain reaction

L. M. Weiss, S. A. Udem, M. Salgo, H. B. Tanowitz, M. Wittner

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

Toxoplasma gondii, an apicomplexan parasite of mammals and birds, is well recognized as a cause of encephalitis in AIDS patients and as a cause of congenital infections. The polymerase chain reaction (PCR) and toxoplasma cDNA clones were used to diagnose T. gondii infection in an acute murine model of toxoplasmosis. Diagnosis of tissue infection by Southern blot hybridization with cDNA clones of T. gondii was possible within 5 days of infection. This technique could detect as few as 10,000 organisms. Specific T. gondii gene amplification by PCR using the primers 5'CACACGGTTGTATGTCGGTTTCGCT3' and 5'TCAAGGAGCTCAATGTTACAGCCT3' followed by oligonucleotide hybridization using 5'GCGGTCATTCTCACACCGACGGAGAACCACTTCACTCTCA3' allowed detection of T. gondii in the tissue of mice by day 2 after infection and in the blood of mice by day 5 after infection with RH strain T. gondii. This technique could detect as few as 10 organisms. Thus, these techniques may be useful in the diagnosis of toxoplasmosis.

Original languageEnglish (US)
Pages (from-to)180-186
Number of pages7
JournalJournal of Infectious Diseases
Volume163
Issue number1
DOIs
StatePublished - 1991

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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