Self-assembling peptide nanofiber as a novel culture system for isolated porcine hepatocytes

Nalu Navarro-Alvarez, Alejandro Soto-Gutierrez, Jorge D. Rivas-Carrillo, Yong Chen, Tsuyoshi Yamamoto, Takeshi Yuasa, Haruo Misawa, Jiro Takei, Noriaki Tanaka, Naoya Kobayashi

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Freshly isolated porcine hepatocytes are a very attractive cell source in the cell-based therapies to treat liver failure because of unlimited availability. However, due to the loss of hepatocyte functions in vitro, there is a need to develop a functional culture system to keep the cells metabolically active. Here we compared the effect of a self-assembling peptide nanofiber (SAPNF) as an extracellular matrix (ECM) with collagen type 1 on hepatocyte metabolic and secretion activities following hepatocyte isolation. Isolated porcine hepatocytes were cultured in SAPNF and collagen type I. Morphological assessment at different time points was performed by using SEM and phase contrast microscope. Metabolic and secretion activities were comparatively performed in the groups, by means of ammonia, lidocaine, and diazepam as well as albumin. Hepatocytes cultured on SAPNF revealed a three-dimensional spheroidal formation, thus maintaining cell differentiation status during 2 weeks of culture. On the other hand, hepatocytes in collagen revealed a spread shape, and by day 14 no hepatocyte-like cells were observed, but cells with long shape were present, thus revealing a degree of dedifferentiation in collagen culture. Hepatocytes in SAPNF were capable of drug-metabolizing activities and albumin secretion in higher ratio than those cultured on collagen. The present work clearly demonstrates the usefulness of SAPNF for maintaining differentiated functions of porcine hepatocytes in culture.

Original languageEnglish (US)
Pages (from-to)921-927
Number of pages7
JournalCell Transplantation
Volume15
Issue number10
DOIs
StatePublished - 2006
Externally publishedYes

Fingerprint

Nanofibers
Peptides
Hepatocytes
Swine
Collagen
Collagen Type I
Albumins
Liver Failure
Diazepam
Cell- and Tissue-Based Therapy
Lidocaine
Ammonia
Liver
Extracellular Matrix
Cell Differentiation
Microscopes
Availability

Keywords

  • Extracellular matrix
  • Hepatocyte culture
  • Porcine hepatocytes
  • Self-assembling peptide nanofiber

ASJC Scopus subject areas

  • Cell Biology
  • Transplantation

Cite this

Navarro-Alvarez, N., Soto-Gutierrez, A., Rivas-Carrillo, J. D., Chen, Y., Yamamoto, T., Yuasa, T., ... Kobayashi, N. (2006). Self-assembling peptide nanofiber as a novel culture system for isolated porcine hepatocytes. Cell Transplantation, 15(10), 921-927. https://doi.org/10.3727/000000006783981387

Self-assembling peptide nanofiber as a novel culture system for isolated porcine hepatocytes. / Navarro-Alvarez, Nalu; Soto-Gutierrez, Alejandro; Rivas-Carrillo, Jorge D.; Chen, Yong; Yamamoto, Tsuyoshi; Yuasa, Takeshi; Misawa, Haruo; Takei, Jiro; Tanaka, Noriaki; Kobayashi, Naoya.

In: Cell Transplantation, Vol. 15, No. 10, 2006, p. 921-927.

Research output: Contribution to journalArticle

Navarro-Alvarez, N, Soto-Gutierrez, A, Rivas-Carrillo, JD, Chen, Y, Yamamoto, T, Yuasa, T, Misawa, H, Takei, J, Tanaka, N & Kobayashi, N 2006, 'Self-assembling peptide nanofiber as a novel culture system for isolated porcine hepatocytes', Cell Transplantation, vol. 15, no. 10, pp. 921-927. https://doi.org/10.3727/000000006783981387
Navarro-Alvarez N, Soto-Gutierrez A, Rivas-Carrillo JD, Chen Y, Yamamoto T, Yuasa T et al. Self-assembling peptide nanofiber as a novel culture system for isolated porcine hepatocytes. Cell Transplantation. 2006;15(10):921-927. https://doi.org/10.3727/000000006783981387
Navarro-Alvarez, Nalu ; Soto-Gutierrez, Alejandro ; Rivas-Carrillo, Jorge D. ; Chen, Yong ; Yamamoto, Tsuyoshi ; Yuasa, Takeshi ; Misawa, Haruo ; Takei, Jiro ; Tanaka, Noriaki ; Kobayashi, Naoya. / Self-assembling peptide nanofiber as a novel culture system for isolated porcine hepatocytes. In: Cell Transplantation. 2006 ; Vol. 15, No. 10. pp. 921-927.
@article{66998143cdde4c6e877b6d5851ae8a37,
title = "Self-assembling peptide nanofiber as a novel culture system for isolated porcine hepatocytes",
abstract = "Freshly isolated porcine hepatocytes are a very attractive cell source in the cell-based therapies to treat liver failure because of unlimited availability. However, due to the loss of hepatocyte functions in vitro, there is a need to develop a functional culture system to keep the cells metabolically active. Here we compared the effect of a self-assembling peptide nanofiber (SAPNF) as an extracellular matrix (ECM) with collagen type 1 on hepatocyte metabolic and secretion activities following hepatocyte isolation. Isolated porcine hepatocytes were cultured in SAPNF and collagen type I. Morphological assessment at different time points was performed by using SEM and phase contrast microscope. Metabolic and secretion activities were comparatively performed in the groups, by means of ammonia, lidocaine, and diazepam as well as albumin. Hepatocytes cultured on SAPNF revealed a three-dimensional spheroidal formation, thus maintaining cell differentiation status during 2 weeks of culture. On the other hand, hepatocytes in collagen revealed a spread shape, and by day 14 no hepatocyte-like cells were observed, but cells with long shape were present, thus revealing a degree of dedifferentiation in collagen culture. Hepatocytes in SAPNF were capable of drug-metabolizing activities and albumin secretion in higher ratio than those cultured on collagen. The present work clearly demonstrates the usefulness of SAPNF for maintaining differentiated functions of porcine hepatocytes in culture.",
keywords = "Extracellular matrix, Hepatocyte culture, Porcine hepatocytes, Self-assembling peptide nanofiber",
author = "Nalu Navarro-Alvarez and Alejandro Soto-Gutierrez and Rivas-Carrillo, {Jorge D.} and Yong Chen and Tsuyoshi Yamamoto and Takeshi Yuasa and Haruo Misawa and Jiro Takei and Noriaki Tanaka and Naoya Kobayashi",
year = "2006",
doi = "10.3727/000000006783981387",
language = "English (US)",
volume = "15",
pages = "921--927",
journal = "Cell Transplantation",
issn = "0963-6897",
publisher = "Cognizant Communication Corporation",
number = "10",

}

TY - JOUR

T1 - Self-assembling peptide nanofiber as a novel culture system for isolated porcine hepatocytes

AU - Navarro-Alvarez, Nalu

AU - Soto-Gutierrez, Alejandro

AU - Rivas-Carrillo, Jorge D.

AU - Chen, Yong

AU - Yamamoto, Tsuyoshi

AU - Yuasa, Takeshi

AU - Misawa, Haruo

AU - Takei, Jiro

AU - Tanaka, Noriaki

AU - Kobayashi, Naoya

PY - 2006

Y1 - 2006

N2 - Freshly isolated porcine hepatocytes are a very attractive cell source in the cell-based therapies to treat liver failure because of unlimited availability. However, due to the loss of hepatocyte functions in vitro, there is a need to develop a functional culture system to keep the cells metabolically active. Here we compared the effect of a self-assembling peptide nanofiber (SAPNF) as an extracellular matrix (ECM) with collagen type 1 on hepatocyte metabolic and secretion activities following hepatocyte isolation. Isolated porcine hepatocytes were cultured in SAPNF and collagen type I. Morphological assessment at different time points was performed by using SEM and phase contrast microscope. Metabolic and secretion activities were comparatively performed in the groups, by means of ammonia, lidocaine, and diazepam as well as albumin. Hepatocytes cultured on SAPNF revealed a three-dimensional spheroidal formation, thus maintaining cell differentiation status during 2 weeks of culture. On the other hand, hepatocytes in collagen revealed a spread shape, and by day 14 no hepatocyte-like cells were observed, but cells with long shape were present, thus revealing a degree of dedifferentiation in collagen culture. Hepatocytes in SAPNF were capable of drug-metabolizing activities and albumin secretion in higher ratio than those cultured on collagen. The present work clearly demonstrates the usefulness of SAPNF for maintaining differentiated functions of porcine hepatocytes in culture.

AB - Freshly isolated porcine hepatocytes are a very attractive cell source in the cell-based therapies to treat liver failure because of unlimited availability. However, due to the loss of hepatocyte functions in vitro, there is a need to develop a functional culture system to keep the cells metabolically active. Here we compared the effect of a self-assembling peptide nanofiber (SAPNF) as an extracellular matrix (ECM) with collagen type 1 on hepatocyte metabolic and secretion activities following hepatocyte isolation. Isolated porcine hepatocytes were cultured in SAPNF and collagen type I. Morphological assessment at different time points was performed by using SEM and phase contrast microscope. Metabolic and secretion activities were comparatively performed in the groups, by means of ammonia, lidocaine, and diazepam as well as albumin. Hepatocytes cultured on SAPNF revealed a three-dimensional spheroidal formation, thus maintaining cell differentiation status during 2 weeks of culture. On the other hand, hepatocytes in collagen revealed a spread shape, and by day 14 no hepatocyte-like cells were observed, but cells with long shape were present, thus revealing a degree of dedifferentiation in collagen culture. Hepatocytes in SAPNF were capable of drug-metabolizing activities and albumin secretion in higher ratio than those cultured on collagen. The present work clearly demonstrates the usefulness of SAPNF for maintaining differentiated functions of porcine hepatocytes in culture.

KW - Extracellular matrix

KW - Hepatocyte culture

KW - Porcine hepatocytes

KW - Self-assembling peptide nanofiber

UR - http://www.scopus.com/inward/record.url?scp=33846605588&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846605588&partnerID=8YFLogxK

U2 - 10.3727/000000006783981387

DO - 10.3727/000000006783981387

M3 - Article

C2 - 17299997

AN - SCOPUS:33846605588

VL - 15

SP - 921

EP - 927

JO - Cell Transplantation

JF - Cell Transplantation

SN - 0963-6897

IS - 10

ER -