Selection and characterization of eight phenotypically distinct lines of lectin-resistant chinese hamster ovary cells

Pamela Stanley, Velda Caillibot, Louis Siminovitch

Research output: Contribution to journalArticle

172 Citations (Scopus)

Abstract

Clones resistant to the lectins phytohemagglutinin (PHA), wheat germ agglutinin (WGA), the agglutinin(s) from Lens culinaris (LCA), and ricin (RIC) have been selected from parental auxotrophic Chinese hamster ovary (CHO) cells. The sensitivity to other lectins of these cells and of CHO cells resistant to concanavalin A (ConA) has been determined, and their activity of UDP-N-acetyl-glucosamine glycoprotein N-acetyl-glucosaminyltransferase (GlcNAc-T) has been measured. At least 8 different phenotypes have been identified on the basis of this analysis, and complementation between 2 of them has been demonstrated.

Original languageEnglish (US)
Pages (from-to)121-128
Number of pages8
JournalCell
Volume6
Issue number2
DOIs
StatePublished - 1975
Externally publishedYes

Fingerprint

Cricetulus
Lectins
Ovary
Cells
Ricin
Wheat Germ Agglutinins
Uridine Diphosphate
Agglutinins
Glucosamine
Phytohemagglutinins
Concanavalin A
Lenses
Glycoproteins
Clone Cells
Phenotype

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Selection and characterization of eight phenotypically distinct lines of lectin-resistant chinese hamster ovary cells. / Stanley, Pamela; Caillibot, Velda; Siminovitch, Louis.

In: Cell, Vol. 6, No. 2, 1975, p. 121-128.

Research output: Contribution to journalArticle

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