TY - JOUR
T1 - Selection and characterization of eight phenotypically distinct lines of lectin-resistant chinese hamster ovary cells
AU - Stanley, Pamela
AU - Caillibot, Velda
AU - Siminovitch, Louis
N1 - Funding Information:
The authors wish to thank Nancy Stokoe for excellent assistance, and Stephen Rothstein and Shirleen Roeder help in some of the experiments reported in this paper. search was supported by the Medical Research Council of the National Cancer Institute of Canada, and the NIH.
PY - 1975/10
Y1 - 1975/10
N2 - Clones resistant to the lectins phytohemagglutinin (PHA), wheat germ agglutinin (WGA), the agglutinin(s) from Lens culinaris (LCA), and ricin (RIC) have been selected from parental auxotrophic Chinese hamster ovary (CHO) cells. The sensitivity to other lectins of these cells and of CHO cells resistant to concanavalin A (ConA) has been determined, and their activity of UDP-N-acetyl-glucosamine glycoprotein N-acetyl-glucosaminyltransferase (GlcNAc-T) has been measured. At least 8 different phenotypes have been identified on the basis of this analysis, and complementation between 2 of them has been demonstrated.
AB - Clones resistant to the lectins phytohemagglutinin (PHA), wheat germ agglutinin (WGA), the agglutinin(s) from Lens culinaris (LCA), and ricin (RIC) have been selected from parental auxotrophic Chinese hamster ovary (CHO) cells. The sensitivity to other lectins of these cells and of CHO cells resistant to concanavalin A (ConA) has been determined, and their activity of UDP-N-acetyl-glucosamine glycoprotein N-acetyl-glucosaminyltransferase (GlcNAc-T) has been measured. At least 8 different phenotypes have been identified on the basis of this analysis, and complementation between 2 of them has been demonstrated.
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U2 - 10.1016/0092-8674(75)90002-1
DO - 10.1016/0092-8674(75)90002-1
M3 - Article
C2 - 1182798
AN - SCOPUS:0016711695
VL - 6
SP - 121
EP - 128
JO - Cell
JF - Cell
SN - 0092-8674
IS - 2
ER -