Screening for DNA adducts by data-dependent constant neutral loss-triple stage mass spectrometry with a linear quadrupole ion trap mass spectrometer

Erin E. Bessette, Angela K. Goodenough, Sophie Langouët, Isil Yasa, Ivan D. Kozekov, Simon D. Spivack, Robert J. Turesky

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

A two-dimensional linear quadrupole ion trap mass spectrometer (LIT/MS) was employed to simultaneously screen for DNA adducts of environmental, dietary, and endogenous genotoxicants, by data-dependent constant neutral loss scanning followed by triple-stage mass spectrometry (CNL-MS 3). The loss of the deoxyribose (dR) from the protonated DNA adducts ([M + H - 116] +) in the MS/MS scan mode triggered the acquisition of MS 3 production spectra of the aglycone adducts [BH 2] +. Five DNA adducts of the tobacco carcinogen 4-aminobiphenyl (4-ABP) were detected in human hepatocytes treated with 4-ABP, and three DNA adducts of the cooked-meat carcinogen 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx) were identified in the livers of rats exposed to MeIQx, by the CNL-MS 3 scan mode. Buccal cell DNA from tobacco smokers was screened for DNA adducts of various classes of carcinogens in tobacco smoke including 4-ABP, 2-amino-9H-pyrido[2,3- b]indole (AαC), and benzo[a]pyrene (BaP); the cooked-meat carcinogens MeIQx, AαC, and 2-amino-1-methyl-6-phenylmidazo[4,5-b]pyridine (PhIP); and the lipid peroxidation products acrolein (AC) and trans-4-hydroxynonenal (HNE). The CNL-MS 3 scanning technique can be used to simultaneously screen for multiple DNA adducts derived from different classes of carcinogens, at levels of adduct modification approaching 1 adduct per 10 8 unmodified DNA bases, when 10 μg of DNA is employed for the assay.

Original languageEnglish (US)
Pages (from-to)809-819
Number of pages11
JournalAnalytical Chemistry
Volume81
Issue number2
DOIs
StatePublished - Jan 15 2009

Fingerprint

DNA Adducts
Mass spectrometers
Mass spectrometry
Screening
Carcinogens
Ions
Acrolein
Tobacco
2-amino-3,8-dimethylimidazo(4,5-f)quinoxaline
Meats
DNA
Deoxyribose
Scanning
Benzo(a)pyrene
Smoke
Liver
Rats
Assays
Lipids
4-biphenylamine

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Screening for DNA adducts by data-dependent constant neutral loss-triple stage mass spectrometry with a linear quadrupole ion trap mass spectrometer. / Bessette, Erin E.; Goodenough, Angela K.; Langouët, Sophie; Yasa, Isil; Kozekov, Ivan D.; Spivack, Simon D.; Turesky, Robert J.

In: Analytical Chemistry, Vol. 81, No. 2, 15.01.2009, p. 809-819.

Research output: Contribution to journalArticle

Bessette, Erin E. ; Goodenough, Angela K. ; Langouët, Sophie ; Yasa, Isil ; Kozekov, Ivan D. ; Spivack, Simon D. ; Turesky, Robert J. / Screening for DNA adducts by data-dependent constant neutral loss-triple stage mass spectrometry with a linear quadrupole ion trap mass spectrometer. In: Analytical Chemistry. 2009 ; Vol. 81, No. 2. pp. 809-819.
@article{3f54ad1a07b648cd9dcfa89e77f38214,
title = "Screening for DNA adducts by data-dependent constant neutral loss-triple stage mass spectrometry with a linear quadrupole ion trap mass spectrometer",
abstract = "A two-dimensional linear quadrupole ion trap mass spectrometer (LIT/MS) was employed to simultaneously screen for DNA adducts of environmental, dietary, and endogenous genotoxicants, by data-dependent constant neutral loss scanning followed by triple-stage mass spectrometry (CNL-MS 3). The loss of the deoxyribose (dR) from the protonated DNA adducts ([M + H - 116] +) in the MS/MS scan mode triggered the acquisition of MS 3 production spectra of the aglycone adducts [BH 2] +. Five DNA adducts of the tobacco carcinogen 4-aminobiphenyl (4-ABP) were detected in human hepatocytes treated with 4-ABP, and three DNA adducts of the cooked-meat carcinogen 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx) were identified in the livers of rats exposed to MeIQx, by the CNL-MS 3 scan mode. Buccal cell DNA from tobacco smokers was screened for DNA adducts of various classes of carcinogens in tobacco smoke including 4-ABP, 2-amino-9H-pyrido[2,3- b]indole (AαC), and benzo[a]pyrene (BaP); the cooked-meat carcinogens MeIQx, AαC, and 2-amino-1-methyl-6-phenylmidazo[4,5-b]pyridine (PhIP); and the lipid peroxidation products acrolein (AC) and trans-4-hydroxynonenal (HNE). The CNL-MS 3 scanning technique can be used to simultaneously screen for multiple DNA adducts derived from different classes of carcinogens, at levels of adduct modification approaching 1 adduct per 10 8 unmodified DNA bases, when 10 μg of DNA is employed for the assay.",
author = "Bessette, {Erin E.} and Goodenough, {Angela K.} and Sophie Langou{\"e}t and Isil Yasa and Kozekov, {Ivan D.} and Spivack, {Simon D.} and Turesky, {Robert J.}",
year = "2009",
month = "1",
day = "15",
doi = "10.1021/ac802096p",
language = "English (US)",
volume = "81",
pages = "809--819",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "2",

}

TY - JOUR

T1 - Screening for DNA adducts by data-dependent constant neutral loss-triple stage mass spectrometry with a linear quadrupole ion trap mass spectrometer

AU - Bessette, Erin E.

AU - Goodenough, Angela K.

AU - Langouët, Sophie

AU - Yasa, Isil

AU - Kozekov, Ivan D.

AU - Spivack, Simon D.

AU - Turesky, Robert J.

PY - 2009/1/15

Y1 - 2009/1/15

N2 - A two-dimensional linear quadrupole ion trap mass spectrometer (LIT/MS) was employed to simultaneously screen for DNA adducts of environmental, dietary, and endogenous genotoxicants, by data-dependent constant neutral loss scanning followed by triple-stage mass spectrometry (CNL-MS 3). The loss of the deoxyribose (dR) from the protonated DNA adducts ([M + H - 116] +) in the MS/MS scan mode triggered the acquisition of MS 3 production spectra of the aglycone adducts [BH 2] +. Five DNA adducts of the tobacco carcinogen 4-aminobiphenyl (4-ABP) were detected in human hepatocytes treated with 4-ABP, and three DNA adducts of the cooked-meat carcinogen 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx) were identified in the livers of rats exposed to MeIQx, by the CNL-MS 3 scan mode. Buccal cell DNA from tobacco smokers was screened for DNA adducts of various classes of carcinogens in tobacco smoke including 4-ABP, 2-amino-9H-pyrido[2,3- b]indole (AαC), and benzo[a]pyrene (BaP); the cooked-meat carcinogens MeIQx, AαC, and 2-amino-1-methyl-6-phenylmidazo[4,5-b]pyridine (PhIP); and the lipid peroxidation products acrolein (AC) and trans-4-hydroxynonenal (HNE). The CNL-MS 3 scanning technique can be used to simultaneously screen for multiple DNA adducts derived from different classes of carcinogens, at levels of adduct modification approaching 1 adduct per 10 8 unmodified DNA bases, when 10 μg of DNA is employed for the assay.

AB - A two-dimensional linear quadrupole ion trap mass spectrometer (LIT/MS) was employed to simultaneously screen for DNA adducts of environmental, dietary, and endogenous genotoxicants, by data-dependent constant neutral loss scanning followed by triple-stage mass spectrometry (CNL-MS 3). The loss of the deoxyribose (dR) from the protonated DNA adducts ([M + H - 116] +) in the MS/MS scan mode triggered the acquisition of MS 3 production spectra of the aglycone adducts [BH 2] +. Five DNA adducts of the tobacco carcinogen 4-aminobiphenyl (4-ABP) were detected in human hepatocytes treated with 4-ABP, and three DNA adducts of the cooked-meat carcinogen 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx) were identified in the livers of rats exposed to MeIQx, by the CNL-MS 3 scan mode. Buccal cell DNA from tobacco smokers was screened for DNA adducts of various classes of carcinogens in tobacco smoke including 4-ABP, 2-amino-9H-pyrido[2,3- b]indole (AαC), and benzo[a]pyrene (BaP); the cooked-meat carcinogens MeIQx, AαC, and 2-amino-1-methyl-6-phenylmidazo[4,5-b]pyridine (PhIP); and the lipid peroxidation products acrolein (AC) and trans-4-hydroxynonenal (HNE). The CNL-MS 3 scanning technique can be used to simultaneously screen for multiple DNA adducts derived from different classes of carcinogens, at levels of adduct modification approaching 1 adduct per 10 8 unmodified DNA bases, when 10 μg of DNA is employed for the assay.

UR - http://www.scopus.com/inward/record.url?scp=60549090590&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=60549090590&partnerID=8YFLogxK

U2 - 10.1021/ac802096p

DO - 10.1021/ac802096p

M3 - Article

C2 - 19086795

AN - SCOPUS:60549090590

VL - 81

SP - 809

EP - 819

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 2

ER -