Salmonella-macrophage interactions upon manganese supplementation

Praveen Rishi, Natasha Jindal, Sushma Bharrhan, Ram Prakash Tiwari

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Various studies indicate the role of manganese (Mn) in the virulence of pathogens. Salmonella is an intracellular pathogen which is able to multiply within the macrophages. The present study was therefore, designed to assess the effect of Mn supplementation on Salmonella-macrophage interactions particularly in reference to Salmonella virulence and macrophage functions. A 50-fold decrease in the lethal dose 50 (LD50) of Salmonella typhimurium was observed when mice were infected with Salmonella grown in the presence of Mn as compared to the LD50 in the absence of Mn indicating an increase in the virulence of the organism. A significant increase was observed in the levels of superoxide dismutase (SOD) of S. typhimurium grown in presence of manganese. Upon Mn supplementation, macrophage functions were also found to be altered. Decreased phagocytic activity of macrophages interacted with Salmonella was observed in presence of Mn as compared to the activity in the absence of Mn. A significant increase was observed in the extent of lipid peroxidation along with significant decreases in the activities of SOD and catalase as well as nitrite levels of macrophages interacted with S. typhimurium upon supplementation with Mn. These observations indicate that Mn supplementation might have increased the expression of Mn transporters in Salmonella resulting in increased levels of its superoxide dismutase. The altered Salmonella function in turn might have been responsible for inhibiting phagocytosis and impairing the balance between the oxidant and antioxidant profile of macrophages, thus protecting itself by exhibiting exalted virulence.

Original languageEnglish (US)
Pages (from-to)110-119
Number of pages10
JournalBiological Trace Element Research
Volume133
Issue number1
DOIs
StatePublished - Jan 1 2010
Externally publishedYes

Fingerprint

Salmonella
Macrophages
Manganese
Virulence
Salmonella typhimurium
Superoxide Dismutase
Lethal Dose 50
Pathogens
Nitrites
Phagocytosis
Oxidants
Catalase
Lipid Peroxidation
Antioxidants

Keywords

  • Catalase
  • Manganese
  • Nitrite
  • Phagocytic activity
  • Salmonella typhimurium
  • Superoxide dismutase
  • Virulence

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical
  • Inorganic Chemistry

Cite this

Salmonella-macrophage interactions upon manganese supplementation. / Rishi, Praveen; Jindal, Natasha; Bharrhan, Sushma; Tiwari, Ram Prakash.

In: Biological Trace Element Research, Vol. 133, No. 1, 01.01.2010, p. 110-119.

Research output: Contribution to journalArticle

Rishi, Praveen ; Jindal, Natasha ; Bharrhan, Sushma ; Tiwari, Ram Prakash. / Salmonella-macrophage interactions upon manganese supplementation. In: Biological Trace Element Research. 2010 ; Vol. 133, No. 1. pp. 110-119.
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