Rotational order-disorder structure of fluorescent protein FP480

Sergei Pletnev, Kateryna S. Morozova, Vladislav V. Verkhusha, Zbigniew Dauter

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

In the last decade, advances in instrumentation and software development have made crystallography a powerful tool in structural biology. Using this method, structural information can now be acquired from pathological crystals that would have been abandoned in earlier times. In this paper, the order-disorder (OD) structure of fluorescent protein FP480 is discussed. The structure is composed of tetramers with 222 symmetry incorporated into the lattice in two different ways, namely rotated 90° with respect to each other around the crystal c axis, with tetramer axes coincident with crystallo-graphic twofold axes. The random distribution of alternatively oriented tetramers in the crystal creates a rotational OD structure with statistically averaged I422 symmetry, although the presence of very weak and diffuse additional reflections suggests that the randomness is only approximate.

Original languageEnglish (US)
Pages (from-to)906-912
Number of pages7
JournalActa Crystallographica Section D: Biological Crystallography
Volume65
Issue number9
DOIs
StatePublished - 2009

Keywords

  • Fluorescent proteins
  • Order-disorder structures
  • Rotational order-disorder

ASJC Scopus subject areas

  • Structural Biology

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