Roles of H3K27me2 and H3K27me3 Examined during Fate Specification of Embryonic Stem Cells

Aster H. Juan; Stan Wang; Kyung Dae Ko; Hossein Zare; Pei Fang Tsai; Xuesong Feng; Karinna O. Vivanco; Anthony M. Ascoli; Gustavo Gutierrez-Cruz; Jordan Krebs; Simone Sidoli; Adam L. Knight; Roger A. Pedersen; Benjamin A. Garcia; Rafael Casellas; Jizhong Zou; Vittorio Sartorelli

doi:10.1016/j.celrep.2016.09.087

Roles of H3K27me2 and H3K27me3 Examined during Fate Specification of Embryonic Stem Cells

Aster H. Juan, Stan Wang, Kyung Dae Ko, Hossein Zare, Pei Fang Tsai, Xuesong Feng, Karinna O. Vivanco, Anthony M. Ascoli, Gustavo Gutierrez-Cruz, Jordan Krebs, Simone Sidoli, Adam L. Knight, Roger A. Pedersen, Benjamin A. Garcia, Rafael Casellas, Jizhong Zou, Vittorio Sartorelli

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

The polycomb repressive complex 2 (PRC2) methylates lysine 27 of histone H3 (H3K27) through its catalytic subunit Ezh2. PRC2-mediated di- and tri-methylation (H3K27me2/H3K27me3) have been interchangeably associated with gene repression. However, it remains unclear whether these two degrees of H3K27 methylation have different functions. In this study, we have generated isogenic mouse embryonic stem cells (ESCs) with a modified H3K27me2/H3K27me3 ratio. Our findings document dynamic developmental control in the genomic distribution of H3K27me2 and H3K27me3 at regulatory regions in ESCs. They also reveal that modifying the ratio of H3K27me2 and H3K27me3 is sufficient for the acquisition and repression of defined cell lineage transcriptional programs and phenotypes and influences induction of the ESC ground state.

Original language	English (US)
Pages (from-to)	1369-1382
Number of pages	14
Journal	Cell Reports
Volume	17
Issue number	5
DOIs	https://doi.org/10.1016/j.celrep.2016.09.087
State	Published - Oct 25 2016
Externally published	Yes

Keywords

H3K27 methylation
embryonic stem cells
polycomb proteins

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1016/j.celrep.2016.09.087

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Juan, A. H., Wang, S., Ko, K. D., Zare, H., Tsai, P. F., Feng, X., Vivanco, K. O., Ascoli, A. M., Gutierrez-Cruz, G., Krebs, J., Sidoli, S., Knight, A. L., Pedersen, R. A., Garcia, B. A., Casellas, R., Zou, J., & Sartorelli, V. (2016). Roles of H3K27me2 and H3K27me3 Examined during Fate Specification of Embryonic Stem Cells. Cell Reports, 17(5), 1369-1382. https://doi.org/10.1016/j.celrep.2016.09.087

Juan, AH, Wang, S, Ko, KD, Zare, H, Tsai, PF, Feng, X, Vivanco, KO, Ascoli, AM, Gutierrez-Cruz, G, Krebs, J, Sidoli, S, Knight, AL, Pedersen, RA, Garcia, BA, Casellas, R, Zou, J & Sartorelli, V 2016, 'Roles of H3K27me2 and H3K27me3 Examined during Fate Specification of Embryonic Stem Cells', Cell Reports, vol. 17, no. 5, pp. 1369-1382. https://doi.org/10.1016/j.celrep.2016.09.087

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title = "Roles of H3K27me2 and H3K27me3 Examined during Fate Specification of Embryonic Stem Cells",

abstract = "The polycomb repressive complex 2 (PRC2) methylates lysine 27 of histone H3 (H3K27) through its catalytic subunit Ezh2. PRC2-mediated di- and tri-methylation (H3K27me2/H3K27me3) have been interchangeably associated with gene repression. However, it remains unclear whether these two degrees of H3K27 methylation have different functions. In this study, we have generated isogenic mouse embryonic stem cells (ESCs) with a modified H3K27me2/H3K27me3 ratio. Our findings document dynamic developmental control in the genomic distribution of H3K27me2 and H3K27me3 at regulatory regions in ESCs. They also reveal that modifying the ratio of H3K27me2 and H3K27me3 is sufficient for the acquisition and repression of defined cell lineage transcriptional programs and phenotypes and influences induction of the ESC ground state.",

keywords = "H3K27 methylation, embryonic stem cells, polycomb proteins",

author = "Juan, {Aster H.} and Stan Wang and Ko, {Kyung Dae} and Hossein Zare and Tsai, {Pei Fang} and Xuesong Feng and Vivanco, {Karinna O.} and Ascoli, {Anthony M.} and Gustavo Gutierrez-Cruz and Jordan Krebs and Simone Sidoli and Knight, {Adam L.} and Pedersen, {Roger A.} and Garcia, {Benjamin A.} and Rafael Casellas and Jizhong Zou and Vittorio Sartorelli",

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doi = "10.1016/j.celrep.2016.09.087",

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AU - Juan, Aster H.

AU - Wang, Stan

AU - Ko, Kyung Dae

AU - Zare, Hossein

AU - Tsai, Pei Fang

AU - Feng, Xuesong

AU - Vivanco, Karinna O.

AU - Ascoli, Anthony M.

AU - Gutierrez-Cruz, Gustavo

AU - Krebs, Jordan

AU - Sidoli, Simone

AU - Knight, Adam L.

AU - Pedersen, Roger A.

AU - Garcia, Benjamin A.

AU - Casellas, Rafael

AU - Zou, Jizhong

AU - Sartorelli, Vittorio

PY - 2016/10/25

Y1 - 2016/10/25

N2 - The polycomb repressive complex 2 (PRC2) methylates lysine 27 of histone H3 (H3K27) through its catalytic subunit Ezh2. PRC2-mediated di- and tri-methylation (H3K27me2/H3K27me3) have been interchangeably associated with gene repression. However, it remains unclear whether these two degrees of H3K27 methylation have different functions. In this study, we have generated isogenic mouse embryonic stem cells (ESCs) with a modified H3K27me2/H3K27me3 ratio. Our findings document dynamic developmental control in the genomic distribution of H3K27me2 and H3K27me3 at regulatory regions in ESCs. They also reveal that modifying the ratio of H3K27me2 and H3K27me3 is sufficient for the acquisition and repression of defined cell lineage transcriptional programs and phenotypes and influences induction of the ESC ground state.

AB - The polycomb repressive complex 2 (PRC2) methylates lysine 27 of histone H3 (H3K27) through its catalytic subunit Ezh2. PRC2-mediated di- and tri-methylation (H3K27me2/H3K27me3) have been interchangeably associated with gene repression. However, it remains unclear whether these two degrees of H3K27 methylation have different functions. In this study, we have generated isogenic mouse embryonic stem cells (ESCs) with a modified H3K27me2/H3K27me3 ratio. Our findings document dynamic developmental control in the genomic distribution of H3K27me2 and H3K27me3 at regulatory regions in ESCs. They also reveal that modifying the ratio of H3K27me2 and H3K27me3 is sufficient for the acquisition and repression of defined cell lineage transcriptional programs and phenotypes and influences induction of the ESC ground state.

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Roles of H3K27me2 and H3K27me3 Examined during Fate Specification of Embryonic Stem Cells

Abstract

Keywords

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