Role of the murine 3′ enhancers in IGH expression and class switching

S. Saleque, M. Singh, B. K. Birshtein

Research output: Contribution to journalArticle

Abstract

The murine IgH 3′ regulatory region contains four enhancers-hs3A, hs1,2, hs3B and hs4. Various studies have suggested a role for these enhancers in regulating IgH expression and class switching. Here we assess the role of hs3A and hs1,2 in these processes by exploiting a naturally occurring deletion of these enhancers from the expressed allele of the F1 pre-B cell line, 70Z/3. Comparable mu expression in 70Z/3 and 18-81 (which has an intact 3′ region) indicated that hs3A and hs1,2 are not essential for mu expression at the pre-B cell stage. To assess the role of hs3A and hs1,2 in IgH function at the plasma cell stage, we fused 70Z/3 with the NSO plasma cell line. EMSA analysis of transcription factors known to modulate the 3′ enhancers confirmed the NSO-like plasma cell milieu in the 70Z-NSO hybrid, consistent with the activation of 3′ enhancers. The level of mu protein in a 70Z/3-NSO fusion hybridoma was substantially elevated relative to its pre-B parent and almost comparable to that in several mu-producing spleen cell hybridomas. Furthermore, ELISPOT assays showed that the 70Z-NSO hybrid underwent spontaneous class switching at a frequency comparable to most hybridomas. These results indicate that hs3A and hs1,2 are not essential for high levels of IgH expression or spontaneous class switching in a plasma cell line, suggesting that these processes depend either exclusively on hs3B and/or hs4, and/or there is a partial redundancy of 3′ enhancer function.

Original languageEnglish (US)
JournalFASEB Journal
Volume12
Issue number5
StatePublished - Mar 20 1998

Fingerprint

Immunoglobulin Class Switching
plasma cells
Plasma Cells
hybridomas
Hybridomas
Cells
Plasmas
B-Lymphoid Precursor Cells
mice
cell lines
Cell Line
B-lymphocytes
Enzyme-Linked Immunospot Assay
Nucleic Acid Regulatory Sequences
splenocytes
Redundancy
Assays
Transcription Factors
Fusion reactions
Spleen

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Saleque, S., Singh, M., & Birshtein, B. K. (1998). Role of the murine 3′ enhancers in IGH expression and class switching. FASEB Journal, 12(5).

Role of the murine 3′ enhancers in IGH expression and class switching. / Saleque, S.; Singh, M.; Birshtein, B. K.

In: FASEB Journal, Vol. 12, No. 5, 20.03.1998.

Research output: Contribution to journalArticle

Saleque, S, Singh, M & Birshtein, BK 1998, 'Role of the murine 3′ enhancers in IGH expression and class switching', FASEB Journal, vol. 12, no. 5.
Saleque S, Singh M, Birshtein BK. Role of the murine 3′ enhancers in IGH expression and class switching. FASEB Journal. 1998 Mar 20;12(5).
Saleque, S. ; Singh, M. ; Birshtein, B. K. / Role of the murine 3′ enhancers in IGH expression and class switching. In: FASEB Journal. 1998 ; Vol. 12, No. 5.
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