Role of MIP-1β and RANTES in HIV-1 infection of microglia

Inhibition of infection and induction by IFNβ

Ryuhei Kitai, Meng Liang Zhao, Nan Zhang, Liwei L. Hua, Sunhee C. Lee

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Microglia are the major target of HIV-1 infection in the brain. Microglial infection is CD4-dependent, but the role of chemokine receptors CCR5 and CCR3 and their natural ligands in modulating HIV-1 infection in microglia has been questioned. In primary human fetal microglial cultures, we demonstrate that HIV-1 infection of these cells is dependent on CCR5, since an antibody to CCR5 completely blocked productive infection. Anti-CCR3, in contrast, had a smaller inhibitory effect which was not statistically significant. The chemokine ligands for CCR5, RANTES and MIP-1β, also potently inhibited HIV-1 infection in microglia, but the third ligand MIP-1α failed to show inhibition. Interestingly, when microglial cultures were treated with antibodies specific to each of these chemokines, HIV-1 infection was enhanced by anti-RANTES and anti-MIP-1β, but not by anti-MIP-1α. These results demonstrate the presence of endogenous chemokines that act as endogenous inhibitors of HIV-1 infection in microglia. Additionally, IFNβ, a known anti-viral cytokine, also provided potent inhibition of viral infection as well as induction of all three chemokines in microglia. These results suggest the possibility that type I interferon can down-modulate microglial HIV-1 infection in vivo by multiple mechanisms. (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)230-239
Number of pages10
JournalJournal of Neuroimmunology
Volume110
Issue number1-2
DOIs
StatePublished - Oct 2 2000

Fingerprint

Chemokine CCL5
Microglia
HIV Infections
HIV-1
Chemokines
Infection
Ligands
Interferon Type I
Antibodies
Chemokine Receptors
Virus Diseases
Cytokines
Brain

Keywords

  • AIDS
  • Brain
  • Chemokine

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Clinical Neurology
  • Neurology

Cite this

Role of MIP-1β and RANTES in HIV-1 infection of microglia : Inhibition of infection and induction by IFNβ. / Kitai, Ryuhei; Zhao, Meng Liang; Zhang, Nan; Hua, Liwei L.; Lee, Sunhee C.

In: Journal of Neuroimmunology, Vol. 110, No. 1-2, 02.10.2000, p. 230-239.

Research output: Contribution to journalArticle

Kitai, Ryuhei ; Zhao, Meng Liang ; Zhang, Nan ; Hua, Liwei L. ; Lee, Sunhee C. / Role of MIP-1β and RANTES in HIV-1 infection of microglia : Inhibition of infection and induction by IFNβ. In: Journal of Neuroimmunology. 2000 ; Vol. 110, No. 1-2. pp. 230-239.
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