Role of cholesterol in fusion of Semliki Forest virus with membranes

Margaret Kielian, A. Helenius

Research output: Contribution to journalArticle

138 Citations (Scopus)

Abstract

The low pH-triggered membrane fusion activity of Semliki Forest virus is dependent on the presence of cholesterol in the target membrane. When liposomes containing phospholipids and cholesterol analogs were used, fusion activity was observed with steroids which did not have a planar nucleus or an isooctyl side chain at C-17, but fusion activity was not observed when analogs which lacked the 3β-OH group were used. Binding of virus to liposomes at low pH was similarly, but not totally, dependent on the presence of a 3β-OH sterol.

Original languageEnglish (US)
Pages (from-to)281-283
Number of pages3
JournalJournal of Virology
Volume52
Issue number1
StatePublished - 1984
Externally publishedYes

Fingerprint

Semliki Forest virus
Semliki forest virus
Liposomes
Cholesterol
cholesterol
Virus Attachment
Membrane Fusion
Membranes
Sterols
sterols
steroids
Phospholipids
phospholipids
Steroids
viruses

ASJC Scopus subject areas

  • Immunology

Cite this

Role of cholesterol in fusion of Semliki Forest virus with membranes. / Kielian, Margaret; Helenius, A.

In: Journal of Virology, Vol. 52, No. 1, 1984, p. 281-283.

Research output: Contribution to journalArticle

@article{3d30f6849e2c47bd936efec601b70c34,
title = "Role of cholesterol in fusion of Semliki Forest virus with membranes",
abstract = "The low pH-triggered membrane fusion activity of Semliki Forest virus is dependent on the presence of cholesterol in the target membrane. When liposomes containing phospholipids and cholesterol analogs were used, fusion activity was observed with steroids which did not have a planar nucleus or an isooctyl side chain at C-17, but fusion activity was not observed when analogs which lacked the 3β-OH group were used. Binding of virus to liposomes at low pH was similarly, but not totally, dependent on the presence of a 3β-OH sterol.",
author = "Margaret Kielian and A. Helenius",
year = "1984",
language = "English (US)",
volume = "52",
pages = "281--283",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "1",

}

TY - JOUR

T1 - Role of cholesterol in fusion of Semliki Forest virus with membranes

AU - Kielian, Margaret

AU - Helenius, A.

PY - 1984

Y1 - 1984

N2 - The low pH-triggered membrane fusion activity of Semliki Forest virus is dependent on the presence of cholesterol in the target membrane. When liposomes containing phospholipids and cholesterol analogs were used, fusion activity was observed with steroids which did not have a planar nucleus or an isooctyl side chain at C-17, but fusion activity was not observed when analogs which lacked the 3β-OH group were used. Binding of virus to liposomes at low pH was similarly, but not totally, dependent on the presence of a 3β-OH sterol.

AB - The low pH-triggered membrane fusion activity of Semliki Forest virus is dependent on the presence of cholesterol in the target membrane. When liposomes containing phospholipids and cholesterol analogs were used, fusion activity was observed with steroids which did not have a planar nucleus or an isooctyl side chain at C-17, but fusion activity was not observed when analogs which lacked the 3β-OH group were used. Binding of virus to liposomes at low pH was similarly, but not totally, dependent on the presence of a 3β-OH sterol.

UR - http://www.scopus.com/inward/record.url?scp=0021177971&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021177971&partnerID=8YFLogxK

M3 - Article

VL - 52

SP - 281

EP - 283

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 1

ER -