Role of CCL3/MIP-1α and CCL5/RANTES during acute Trypanosoma cruzi infection in rats

Ester Roffê, Fabiano Oliveira, Adriano L S Souza, Vanessa Pinho, Danielle G. Souza, Patrícia R S Souza, Remo C. Russo, Helton C. Santiago, Álvaro J. Romanha, Herbert B. Tanowitz, Jesus G. Valenzuela, Mauro M. Teixeira

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Chagas' disease is caused by Trypanosoma cruzi infection and is characterized by chronic fibrogenic inflammation and heart dysfunction. Chemokines are produced during infection and drive tissue inflammation. In rats, acute infection is characterized by intense myocarditis and regression of inflammation after control of parasitism. We investigated the role of CCL3 and CCL5 during infection by using DNA vaccination encoding for each chemokine separately or simultaneously. MetRANTES treatment was used to evaluate the role of CCR1 and CCR5, the receptors for CCL3 and CCL5. Vaccination with CCL3 or CCL5 increased heart parasitism and decreased local IFN-γ production, but did not influence intensity of inflammation. Simultaneous treatment with both plasmids or treatment with MetRANTES enhanced cardiac inflammation, fibrosis and parasitism. In conclusion, chemokines CCL3 and CCL5 are relevant, but not essential, for control of T. cruzi infection in rats. On the other hand, combined blockade of these chemokines or their receptors enhanced tissue inflammation and fibrosis, clearly contrasting with available data in murine models of T. cruzi infection. These data reinforce the important role of chemokines during T. cruzi infection but suggest that caution must be taken when expanding the therapeutic modulation of the chemokine system in mice to the human infection.

Original languageEnglish (US)
Pages (from-to)669-676
Number of pages8
JournalMicrobes and Infection
Volume12
Issue number8-9
DOIs
StatePublished - Aug 2010

Fingerprint

Chemokine CCL5
Trypanosoma cruzi
Chemokines
Infection
Inflammation
CCR1 Receptors
Vaccination
Fibrosis
CCR5 Receptors
Chemokine CCL3
Chagas Disease
Myocarditis
Therapeutics
Plasmids
DNA

Keywords

  • CCR5
  • Chemokines
  • Myocarditis
  • Trypanosoma cruzi

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Infectious Diseases
  • Medicine(all)

Cite this

Roffê, E., Oliveira, F., Souza, A. L. S., Pinho, V., Souza, D. G., Souza, P. R. S., ... Teixeira, M. M. (2010). Role of CCL3/MIP-1α and CCL5/RANTES during acute Trypanosoma cruzi infection in rats. Microbes and Infection, 12(8-9), 669-676. https://doi.org/10.1016/j.micinf.2010.04.011

Role of CCL3/MIP-1α and CCL5/RANTES during acute Trypanosoma cruzi infection in rats. / Roffê, Ester; Oliveira, Fabiano; Souza, Adriano L S; Pinho, Vanessa; Souza, Danielle G.; Souza, Patrícia R S; Russo, Remo C.; Santiago, Helton C.; Romanha, Álvaro J.; Tanowitz, Herbert B.; Valenzuela, Jesus G.; Teixeira, Mauro M.

In: Microbes and Infection, Vol. 12, No. 8-9, 08.2010, p. 669-676.

Research output: Contribution to journalArticle

Roffê, E, Oliveira, F, Souza, ALS, Pinho, V, Souza, DG, Souza, PRS, Russo, RC, Santiago, HC, Romanha, ÁJ, Tanowitz, HB, Valenzuela, JG & Teixeira, MM 2010, 'Role of CCL3/MIP-1α and CCL5/RANTES during acute Trypanosoma cruzi infection in rats', Microbes and Infection, vol. 12, no. 8-9, pp. 669-676. https://doi.org/10.1016/j.micinf.2010.04.011
Roffê, Ester ; Oliveira, Fabiano ; Souza, Adriano L S ; Pinho, Vanessa ; Souza, Danielle G. ; Souza, Patrícia R S ; Russo, Remo C. ; Santiago, Helton C. ; Romanha, Álvaro J. ; Tanowitz, Herbert B. ; Valenzuela, Jesus G. ; Teixeira, Mauro M. / Role of CCL3/MIP-1α and CCL5/RANTES during acute Trypanosoma cruzi infection in rats. In: Microbes and Infection. 2010 ; Vol. 12, No. 8-9. pp. 669-676.
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abstract = "Chagas' disease is caused by Trypanosoma cruzi infection and is characterized by chronic fibrogenic inflammation and heart dysfunction. Chemokines are produced during infection and drive tissue inflammation. In rats, acute infection is characterized by intense myocarditis and regression of inflammation after control of parasitism. We investigated the role of CCL3 and CCL5 during infection by using DNA vaccination encoding for each chemokine separately or simultaneously. MetRANTES treatment was used to evaluate the role of CCR1 and CCR5, the receptors for CCL3 and CCL5. Vaccination with CCL3 or CCL5 increased heart parasitism and decreased local IFN-γ production, but did not influence intensity of inflammation. Simultaneous treatment with both plasmids or treatment with MetRANTES enhanced cardiac inflammation, fibrosis and parasitism. In conclusion, chemokines CCL3 and CCL5 are relevant, but not essential, for control of T. cruzi infection in rats. On the other hand, combined blockade of these chemokines or their receptors enhanced tissue inflammation and fibrosis, clearly contrasting with available data in murine models of T. cruzi infection. These data reinforce the important role of chemokines during T. cruzi infection but suggest that caution must be taken when expanding the therapeutic modulation of the chemokine system in mice to the human infection.",
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