RING box protein-1 gene involved in flagellar disassembly of Dunaliella salina

Nannan Zhang, Fangxia Guan, Yanting Zhang, Qinghua Li, Yao Xu, Lu Yang, Xiangzhan Zhu, Mingzhi Zhang

Research output: Contribution to journalArticle

Abstract

Ring box protein-1 (RBX1), also called Regulator of Cullins-1 (ROC1), is a key component of SCF (Skp-1, cullins, F-box proteins) E3 ubiquitin ligases, which regulate diverse cellular processes by targeting protein substrates for degradation. Although RBX1 plays an important role in ubiquitination machinery of both prokaryotes and eukaryotes, studies on the RBX1 have not been involved in the unicellular green alga Dunaliella salina. In this study, a full-length RBX1 cDNA fragment of 817 bp was cloned using rapid amplification of cDNA end (RACE) technique. The full-length sequence contained an open reading frame of 411 bp encoding 136 amino acids. The predicted protein had a molecular molar mass of 14.8 kDa and pI of 5.9 with a high degree of homology to RBX1 from Chlamydomonas reinhardtii (92 %). Recombinant RBX1 was expressed in Escherichia coli BL21 and was purified and characterized. The apparent molecular mass of the recombinant protein was approximately 17 kDa, and the optimal induction time and concentration were 3 h and 0.1 mmol/L IPTG, respectively. The predicted 3D structures of RBX1 proteins contained RING-H2 finger domain including “Cys59-X2-Cys62-X30-Cys93-X1-His95-X2-His98-X2-Cys101-X10-Cys112-X2-Cys115.” The expression of RBX1 protein was increased by 132 % during flagellar disassembly and decreased by 76 % during flagellar assembly of D. salina. The expression of RBX1 mRNA had a similar tendency with the expression of RBX1 protein. The results indicated that RBX1 responded to flagellar disassembly of D. salina.

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalFolia Microbiologica
DOIs
StateAccepted/In press - Sep 22 2016
Externally publishedYes

Fingerprint

Proteins
Salinum
Complementary DNA
RING Finger Domains
F-Box Proteins
Isopropyl Thiogalactoside
Chlamydomonas reinhardtii
Chlorophyta
Ubiquitin-Protein Ligases
Ubiquitination
Eukaryota
Recombinant Proteins
Open Reading Frames
Proteolysis
Escherichia coli
Amino Acids
Messenger RNA

ASJC Scopus subject areas

  • Microbiology

Cite this

Zhang, N., Guan, F., Zhang, Y., Li, Q., Xu, Y., Yang, L., ... Zhang, M. (Accepted/In press). RING box protein-1 gene involved in flagellar disassembly of Dunaliella salina. Folia Microbiologica, 1-6. https://doi.org/10.1007/s12223-016-0472-9

RING box protein-1 gene involved in flagellar disassembly of Dunaliella salina. / Zhang, Nannan; Guan, Fangxia; Zhang, Yanting; Li, Qinghua; Xu, Yao; Yang, Lu; Zhu, Xiangzhan; Zhang, Mingzhi.

In: Folia Microbiologica, 22.09.2016, p. 1-6.

Research output: Contribution to journalArticle

Zhang, Nannan ; Guan, Fangxia ; Zhang, Yanting ; Li, Qinghua ; Xu, Yao ; Yang, Lu ; Zhu, Xiangzhan ; Zhang, Mingzhi. / RING box protein-1 gene involved in flagellar disassembly of Dunaliella salina. In: Folia Microbiologica. 2016 ; pp. 1-6.
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abstract = "Ring box protein-1 (RBX1), also called Regulator of Cullins-1 (ROC1), is a key component of SCF (Skp-1, cullins, F-box proteins) E3 ubiquitin ligases, which regulate diverse cellular processes by targeting protein substrates for degradation. Although RBX1 plays an important role in ubiquitination machinery of both prokaryotes and eukaryotes, studies on the RBX1 have not been involved in the unicellular green alga Dunaliella salina. In this study, a full-length RBX1 cDNA fragment of 817 bp was cloned using rapid amplification of cDNA end (RACE) technique. The full-length sequence contained an open reading frame of 411 bp encoding 136 amino acids. The predicted protein had a molecular molar mass of 14.8 kDa and pI of 5.9 with a high degree of homology to RBX1 from Chlamydomonas reinhardtii (92 {\%}). Recombinant RBX1 was expressed in Escherichia coli BL21 and was purified and characterized. The apparent molecular mass of the recombinant protein was approximately 17 kDa, and the optimal induction time and concentration were 3 h and 0.1 mmol/L IPTG, respectively. The predicted 3D structures of RBX1 proteins contained RING-H2 finger domain including “Cys59-X2-Cys62-X30-Cys93-X1-His95-X2-His98-X2-Cys101-X10-Cys112-X2-Cys115.” The expression of RBX1 protein was increased by 132 {\%} during flagellar disassembly and decreased by 76 {\%} during flagellar assembly of D. salina. The expression of RBX1 mRNA had a similar tendency with the expression of RBX1 protein. The results indicated that RBX1 responded to flagellar disassembly of D. salina.",
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