Ribosomal DNA sequence of Nucleospora salmonis hedrick, groff and baxa, 1991 (Microsporea: Enterocytozoonidae): Implications for phylogeny and nomenclature

M. F. Docker, M. L. Kent, D. M L Hervio, J. S. Khattra, Louis M. Weiss, A. Call, R. H. Devlin

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Rules of zoological nomenclature, morphological data, and ribosomal DNA sequence data support the validity of the genus Nucleospora, and its placement in the family Enterocytozoonidae. Although Nucleospora exhibits most of the distinguishing morphological characteristics of the family Enterocytozoonidae Cali and Owen, 1990, the distinctively different hosts (fish and humans, respectively) and sites of development (the nuclei of immature blood cells and the cytoplasm of enterocytes) support the placement of Nucleospora and Enterocytozoon into separate genera. Ribosomal DNA sequence comparisons between Nucleospora salmonis and Enterocytozoon bieneusi showed 19.8% genetic divergence in the large and small subunit regions. Although more inter- and intrageneric comparisons, are needed before percent homology of ribosomal DNA can be used as a criterion for the separation of genera, the genetic divergence between the two species is sufficiently large to deter suppression of the genus Nucleospora as a junior synonym of Enterocytozoon. A polymerase chain reaction test for the detection of N. salmonis in chinook salmon (Oncorhynchus tshawytscha), based on N. salmonis-specific ribosomal DNA sequence, is described.

Original languageEnglish (US)
Pages (from-to)55-60
Number of pages6
JournalJournal of Eukaryotic Microbiology
Volume44
Issue number1
StatePublished - Jan 1997
Externally publishedYes

Fingerprint

Microsporea
Enterocytozoon
Phylogeny
Ribosomal DNA
Terminology
ribosomal DNA
nucleotide sequences
Salmon
phylogeny
Oncorhynchus tshawytscha
genetic variation
Enterocytes
enterocytes
blood cells
Blood Cells
Cytoplasm
Fishes
cytoplasm
polymerase chain reaction
immatures

Keywords

  • Chinook salmon
  • diagnosis
  • Enterocytozoon salmonis
  • parasite
  • polymerase chain reaction

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Applied Microbiology and Biotechnology
  • Agricultural and Biological Sciences (miscellaneous)
  • Microbiology

Cite this

Ribosomal DNA sequence of Nucleospora salmonis hedrick, groff and baxa, 1991 (Microsporea : Enterocytozoonidae): Implications for phylogeny and nomenclature. / Docker, M. F.; Kent, M. L.; Hervio, D. M L; Khattra, J. S.; Weiss, Louis M.; Call, A.; Devlin, R. H.

In: Journal of Eukaryotic Microbiology, Vol. 44, No. 1, 01.1997, p. 55-60.

Research output: Contribution to journalArticle

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