Retention of antidiuretic hormone-induced particle aggregates by luminal membranes separated from toad bladder epithelial cells

R. M. Hays, J. Bourguet, Birgit H. Satir, N. Franki, J. Rapoport

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Aggregates of intramembrane particles appear in the luminal membranes of renal collecting duct and amphibian bladder cells after stimulation by antidiuretic hormone (ADH). We undertook this freeze-fracture study to determine whether particle aggregates, once in place, remain in the luminal membrane of the amphibian bladder after the membrane is physically separated from the rest of the cell. We found that the aggregates do remain in high yield in isolated membranes stabilized with a bifunctional imidoester (DTBP) followed by fixation with glutaraldehyde,or unfixed but stabilized with DTBP. These findings support the view that the particles are intrinsic membrane components and that their organization in the form of aggregates does not depend on the presence of the intact cell. In addition, the availability of isolated membranes containing particle aggregates provides a starting point for the isolation of the water-conducting proteins.

Original languageEnglish (US)
Pages (from-to)237-241
Number of pages5
JournalJournal of Cell Biology
Volume92
Issue number1
DOIs
StatePublished - 1982

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Vasopressins
Anura
Urinary Bladder
Epithelial Cells
Membranes
Amphibians
Imidoesters
Glutaral
Kidney
Water
Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

Retention of antidiuretic hormone-induced particle aggregates by luminal membranes separated from toad bladder epithelial cells. / Hays, R. M.; Bourguet, J.; Satir, Birgit H.; Franki, N.; Rapoport, J.

In: Journal of Cell Biology, Vol. 92, No. 1, 1982, p. 237-241.

Research output: Contribution to journalArticle

Hays, R. M. ; Bourguet, J. ; Satir, Birgit H. ; Franki, N. ; Rapoport, J. / Retention of antidiuretic hormone-induced particle aggregates by luminal membranes separated from toad bladder epithelial cells. In: Journal of Cell Biology. 1982 ; Vol. 92, No. 1. pp. 237-241.
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