Resonance Raman study of the dark-adapted form of the purple membrane protein

B. Aton, A. G. Doukas, R. H. Callender, B. Becher, T. G. Ebrey

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

The resonance Raman spectrum of the dark-adapted form of the purple membrane protein (bacteriorhodopsin) has been obtained and is compared to the light-adapted pigment and model chromophore spectra. As in the light-adapted form, the chromophore-protein linkage is found to be a protonated Schiff base. Electron delocalization appears to play the dominant role in color regulation. The dark-adapted spectrum indicates a conformation closer to 13-cis than the light-adapted spectrum.

Original languageEnglish (US)
Pages (from-to)424-428
Number of pages5
JournalBBA - Protein Structure
Volume576
Issue number2
DOIs
Publication statusPublished - Feb 26 1979
Externally publishedYes

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Keywords

  • (Purple membrane)
  • Bacteriorhodopsin
  • Chromaphore-protein linkage
  • Dark-adapted spectrum
  • Raman resonance

ASJC Scopus subject areas

  • Medicine(all)

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