Resonance Raman spectroscopy of an ultraviolet-sensitive insect rhodopsin

C. Pande, Robert Callender, P. Rath, Hua Deng, J. Schwemer

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

We present the first visual pigment resonance Raman spectra from the UV-sensitive eyes of an insect, Ascalaphus macaronius (owlfly). This pigment contains 11-cis-retinal as the chromophore. Raman data have been obtained for the acid metarhodopsin at 10 °C in both H2O and D2O. The C = N stretching mode at 1660 cm-1 in H2O shifts to 1631 cm-1 upon deuteriation of the sample, clearly showing a protonated Schiff base linkage between the chromophore and the protein. The structure-sensitive fingerprint region shows similarities to the all-trans-protonated Schiff base of model retinal chromophores, as well as to the octopus acid metarhodopsin and bovine metarhodopsin I. Although spectra measured at -100 °C with 406.7-nm excitation, to enhance scattering from rhodopsin (λmax 345 nm), contain a significant contribution from a small amount of contaminants [cytochrome(s) and/or accessory pigment] in the sample, the C = N stretch at 1664 cm-1 suggests a protonated Schiff base linkage between the chromophore and the protein in rhodopsin as well. For comparison, this mode also appears at ∼1660 cm-1 in both the vertebrate (bovine) and the invertebrate (octopus) rhodopsins. These data are particularly interesting since the absorption maximum of 345 nm for rhodopsin might be expected to originate from an unprotonated Schiff base linkage. That the Schiff base linkage in the owlfly rhodopsin, like in bovine and in octopus, is protonated suggests that a charged chromophore is essential to visual transduction.

Original languageEnglish (US)
Pages (from-to)7426-7430
Number of pages5
JournalBiochemistry
Volume26
Issue number23
StatePublished - 1987
Externally publishedYes

Fingerprint

Rhodopsin
Schiff Bases
Raman Spectrum Analysis
Chromophores
Insects
Raman spectroscopy
Octopodiformes
Pigments
Retinaldehyde
Acids
Retinal Pigments
Accessories
Dermatoglyphics
Invertebrates
Cytochromes
Stretching
Vertebrates
Raman scattering
Proteins
Scattering

ASJC Scopus subject areas

  • Biochemistry

Cite this

Resonance Raman spectroscopy of an ultraviolet-sensitive insect rhodopsin. / Pande, C.; Callender, Robert; Rath, P.; Deng, Hua; Schwemer, J.

In: Biochemistry, Vol. 26, No. 23, 1987, p. 7426-7430.

Research output: Contribution to journalArticle

Pande, C, Callender, R, Rath, P, Deng, H & Schwemer, J 1987, 'Resonance Raman spectroscopy of an ultraviolet-sensitive insect rhodopsin', Biochemistry, vol. 26, no. 23, pp. 7426-7430.
Pande, C. ; Callender, Robert ; Rath, P. ; Deng, Hua ; Schwemer, J. / Resonance Raman spectroscopy of an ultraviolet-sensitive insect rhodopsin. In: Biochemistry. 1987 ; Vol. 26, No. 23. pp. 7426-7430.
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