Resonance Raman characterization of soluble guanylate cyclase expressed from baculovirus

Baochen Fan, Gopa Gupta, Robert S. Danziger, Joel M. Friedman, Denis L. Rousseau

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Resonance Raman spectra of the α1β1 isoform of bovine lung soluble guanylate cyclase expressed from baculovirus have been measured. The spectra show that the ferric heme is five-coordinate high spin whereas the ferrous heme in the absence of added exogenous ligands is a mixture of six-coordinate low spin and five-coordinate high spin. In the Fe-CO-derivative, the correlation between the Fe-CO frequency (497 cm-1) and the C-O frequency (1959 cm-1) demonstrates that the proximal ligand in our preparation is histidine. The Fe-NO stretching frequency (found at 520 cm-1) and other spectral features of the ferrous Fe-NO-bound sGC are similar to those reported by Deinum et al. (1) and Yu et al. (2). These data indicate that although large preparation-dependent differences in the occupancy of the distal pocket exist, all the preparations have the same proximal histidine ligation and share the same mechanism of activation by NO.

Original languageEnglish (US)
Pages (from-to)1178-1184
Number of pages7
JournalBiochemistry
Volume37
Issue number5
DOIs
StatePublished - Feb 3 1998

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Baculoviridae
Guanylate Cyclase
Carbon Monoxide
Heme
Histidine
Ligands
Stretching
Ligation
Raman scattering
Protein Isoforms
Chemical activation
Derivatives
Lung
Soluble Guanylyl Cyclase

ASJC Scopus subject areas

  • Biochemistry

Cite this

Resonance Raman characterization of soluble guanylate cyclase expressed from baculovirus. / Fan, Baochen; Gupta, Gopa; Danziger, Robert S.; Friedman, Joel M.; Rousseau, Denis L.

In: Biochemistry, Vol. 37, No. 5, 03.02.1998, p. 1178-1184.

Research output: Contribution to journalArticle

Fan, Baochen ; Gupta, Gopa ; Danziger, Robert S. ; Friedman, Joel M. ; Rousseau, Denis L. / Resonance Raman characterization of soluble guanylate cyclase expressed from baculovirus. In: Biochemistry. 1998 ; Vol. 37, No. 5. pp. 1178-1184.
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