TY - JOUR
T1 - Requirement of the familial Alzheimer's disease gene PS2 for apoptosis. Opposing effect of ALG-3
AU - Vito, Pasquale
AU - Wolozin, Benjamin
AU - Kelly Ganjei, J.
AU - Iwasaki, Katsunori
AU - Lacaná, Emanuela
AU - D'Adamio, Luciano
PY - 1996
Y1 - 1996
N2 - ALG-3, a truncated mouse homologue of the chromosome 1 familial Alzheimer's disease gene PS2, rescues T hybridoma 3DO cells from T-cell receptor-induced apoptosis by inhibiting Fas ligand induction and Fas signaling. Here we show that ALG-3 transfected 3DO cells express a COOH- terminal PS2 polypeptide. Overexpression of PS2 in ALG-3 transfected 3DO cells reconstitutes sensitivity to receptor-induced cell death, suggesting that the artificial PS2 polypeptide functions as a dominant negative mutant of PS2. ALG-3 and antisense PS2 protect PC12 cells from glutamate-induced apoptosis but not from death induced by hydrogen peroxide or the free radical MPP+. Thus, the PS2 gene is required for some forms of cell death in diverse cell types, and its function is opposed by ALG-3.
AB - ALG-3, a truncated mouse homologue of the chromosome 1 familial Alzheimer's disease gene PS2, rescues T hybridoma 3DO cells from T-cell receptor-induced apoptosis by inhibiting Fas ligand induction and Fas signaling. Here we show that ALG-3 transfected 3DO cells express a COOH- terminal PS2 polypeptide. Overexpression of PS2 in ALG-3 transfected 3DO cells reconstitutes sensitivity to receptor-induced cell death, suggesting that the artificial PS2 polypeptide functions as a dominant negative mutant of PS2. ALG-3 and antisense PS2 protect PC12 cells from glutamate-induced apoptosis but not from death induced by hydrogen peroxide or the free radical MPP+. Thus, the PS2 gene is required for some forms of cell death in diverse cell types, and its function is opposed by ALG-3.
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U2 - 10.1074/jbc.271.49.31025
DO - 10.1074/jbc.271.49.31025
M3 - Article
C2 - 8940094
AN - SCOPUS:0029856850
SN - 0021-9258
VL - 271
SP - 31025
EP - 31028
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 49
ER -