Replete vitamin D stores predict reproductive success following in vitro fertilization

Sebiha Ozkan, Sangita K. Jindal, Keri Greenseid, Jun Shu, Gohar Zeitlian, Cheryl Hickmon, Lubna Pal

Research output: Contribution to journalArticle

170 Citations (Scopus)

Abstract

Objective: To determine whether 25OH-D levels in the follicular fluid (FF) of infertile women undergoing IVF demonstrate a relationship with IVF cycle parameters and outcome, hypothesizing that levels of 25OH-D in body fluids are reflective of vitamin repletion status. Design: Prospective cohort study. Setting: Academic tertiary care center. Patient(s): Eighty-four infertile women undergoing IVF. Intervention(s): Follicular fluid from follicles ≥14mm; serum (n = 10) and FF levels of 25OH-D. Main Outcome Measure(s): Clinical pregnancy (CP), defined as evidence of intrauterine gestation sac on ultrasound, following IVF; IVF cycle parameters. Result(s): Serum and FF levels of 25OH-D were highly correlated (r = 0.94). In a predominantly Caucasian population (66%), significantly lower FF 25OH-D levels were noted in Black versus non-Black patients. Significant inverse correlations were seen between FF 25OH-D levels and body mass index (r = -0.25). Significantly higher CP and implantation rates were observed across tertiles of FF25OH-D; patients achieving CP following IVF (n = 26) exhibited significantly higher FF levels of 25OH-D. Multivariable logistic regression analysis confirmed FF 25OH-D levels as an independent predictor to success of an IVF cycle; adjusting for age, body mass index, ethnicity, and number of embryos transferred, each ng/mL increase in FF 25OH-D increased the likelihood for achieving CP by 6%. Conclusion(s): Our findings that women with higher vitamin D level in their serum and FF are significantly more likely to achieve CP following IVF-embryo transfer are novel. A potential for benefit of vitamin D supplementation on treatment success in infertile patients undergoing IVF is suggested and merits further investigation.

Original languageEnglish (US)
Pages (from-to)1314-1319
Number of pages6
JournalFertility and Sterility
Volume94
Issue number4
DOIs
StatePublished - Sep 2010

Fingerprint

Follicular Fluid
Fertilization in Vitro
Vitamin D
Pregnancy
Body Mass Index
Serum
Gestational Sac
Embryo Transfer
Body Fluids
Pregnancy Rate
Tertiary Care Centers
Vitamins
Cohort Studies
Embryonic Structures
Logistic Models
Regression Analysis
Outcome Assessment (Health Care)
Prospective Studies

Keywords

  • 25OH-D
  • clinical pregnancy
  • follicular fluid
  • in vitro fertilization
  • infertility
  • Vitamin D

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Reproductive Medicine
  • Medicine(all)

Cite this

Replete vitamin D stores predict reproductive success following in vitro fertilization. / Ozkan, Sebiha; Jindal, Sangita K.; Greenseid, Keri; Shu, Jun; Zeitlian, Gohar; Hickmon, Cheryl; Pal, Lubna.

In: Fertility and Sterility, Vol. 94, No. 4, 09.2010, p. 1314-1319.

Research output: Contribution to journalArticle

Ozkan, Sebiha ; Jindal, Sangita K. ; Greenseid, Keri ; Shu, Jun ; Zeitlian, Gohar ; Hickmon, Cheryl ; Pal, Lubna. / Replete vitamin D stores predict reproductive success following in vitro fertilization. In: Fertility and Sterility. 2010 ; Vol. 94, No. 4. pp. 1314-1319.
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abstract = "Objective: To determine whether 25OH-D levels in the follicular fluid (FF) of infertile women undergoing IVF demonstrate a relationship with IVF cycle parameters and outcome, hypothesizing that levels of 25OH-D in body fluids are reflective of vitamin repletion status. Design: Prospective cohort study. Setting: Academic tertiary care center. Patient(s): Eighty-four infertile women undergoing IVF. Intervention(s): Follicular fluid from follicles ≥14mm; serum (n = 10) and FF levels of 25OH-D. Main Outcome Measure(s): Clinical pregnancy (CP), defined as evidence of intrauterine gestation sac on ultrasound, following IVF; IVF cycle parameters. Result(s): Serum and FF levels of 25OH-D were highly correlated (r = 0.94). In a predominantly Caucasian population (66{\%}), significantly lower FF 25OH-D levels were noted in Black versus non-Black patients. Significant inverse correlations were seen between FF 25OH-D levels and body mass index (r = -0.25). Significantly higher CP and implantation rates were observed across tertiles of FF25OH-D; patients achieving CP following IVF (n = 26) exhibited significantly higher FF levels of 25OH-D. Multivariable logistic regression analysis confirmed FF 25OH-D levels as an independent predictor to success of an IVF cycle; adjusting for age, body mass index, ethnicity, and number of embryos transferred, each ng/mL increase in FF 25OH-D increased the likelihood for achieving CP by 6{\%}. Conclusion(s): Our findings that women with higher vitamin D level in their serum and FF are significantly more likely to achieve CP following IVF-embryo transfer are novel. A potential for benefit of vitamin D supplementation on treatment success in infertile patients undergoing IVF is suggested and merits further investigation.",
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AB - Objective: To determine whether 25OH-D levels in the follicular fluid (FF) of infertile women undergoing IVF demonstrate a relationship with IVF cycle parameters and outcome, hypothesizing that levels of 25OH-D in body fluids are reflective of vitamin repletion status. Design: Prospective cohort study. Setting: Academic tertiary care center. Patient(s): Eighty-four infertile women undergoing IVF. Intervention(s): Follicular fluid from follicles ≥14mm; serum (n = 10) and FF levels of 25OH-D. Main Outcome Measure(s): Clinical pregnancy (CP), defined as evidence of intrauterine gestation sac on ultrasound, following IVF; IVF cycle parameters. Result(s): Serum and FF levels of 25OH-D were highly correlated (r = 0.94). In a predominantly Caucasian population (66%), significantly lower FF 25OH-D levels were noted in Black versus non-Black patients. Significant inverse correlations were seen between FF 25OH-D levels and body mass index (r = -0.25). Significantly higher CP and implantation rates were observed across tertiles of FF25OH-D; patients achieving CP following IVF (n = 26) exhibited significantly higher FF levels of 25OH-D. Multivariable logistic regression analysis confirmed FF 25OH-D levels as an independent predictor to success of an IVF cycle; adjusting for age, body mass index, ethnicity, and number of embryos transferred, each ng/mL increase in FF 25OH-D increased the likelihood for achieving CP by 6%. Conclusion(s): Our findings that women with higher vitamin D level in their serum and FF are significantly more likely to achieve CP following IVF-embryo transfer are novel. A potential for benefit of vitamin D supplementation on treatment success in infertile patients undergoing IVF is suggested and merits further investigation.

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