Repetitive Elements Trigger RIG-I-like Receptor Signaling that Regulates the Emergence of Hematopoietic Stem and Progenitor Cells

Stylianos Lefkopoulos, Aikaterini Polyzou, Marta Derecka, Veronica Bergo, Thomas Clapes, Pierre Cauchy, Carolina Jerez-Longres, Megumi Onishi-Seebacher, Na Yin, Natalia Adriana Martagon-Calderón, Kathryn S. Potts, Lhéanna Klaeylé, Feng Liu, Teresa V. Bowman, Thomas Jenuwein, Maria Caterina Mione, Eirini Trompouki

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Inflammatory signaling is required for hematopoietic stem and progenitor cell (HSPC) development. Here, we studied the involvement of RIG-I-like receptors (RLRs) in HSPC formation. Rig-I or Mda5 deficiency impaired, while Lgp2 deficiency enhanced, HSPC emergence in zebrafish embryos. Rig-I or Mda5 deficiency reduced HSPC numbers by inhibiting inflammatory signals that were in turn enhanced in Lgp2 deficient embryos. Simultaneous reduction of Lgp2 and either Rig-I or Mda5 rescued inflammatory signals and HSPC numbers. Modulating the expression of the signaling mediator Traf6 in RLR deficient embryos restored HSPC numbers. Repetitive element transcripts could be detected in hemogenic endothelial cells and HSPCs, suggesting a role as RLR ligands. Indeed, ectopic expression of repetitive elements enhanced HSPC formation in wild-type, but not in Rig-I or Mda5 deficient embryos. Manipulation of RLR expression in mouse fetal liver HSPCs indicated functional conservation among species. Thus, repetitive elements transcribed during development drive RLR-mediated inflammatory signals that regulate HSPC formation.

Original languageEnglish (US)
Pages (from-to)934-951.e9
JournalImmunity
Volume53
Issue number5
DOIs
StatePublished - Nov 17 2020

Keywords

  • HSCs
  • RIG-I-like receptors
  • development
  • hematopoiesis
  • hematopoietic stem cells
  • inflammation
  • innate immune receptors
  • repetitive elements
  • transposable elements
  • zebrafish

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

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