Repeated administrations of carbon nanotubes in male mice cause reversible testis damage without affecting fertility

Yuhong Bai, Yi Zhang, Jingping Zhang, Qingxin Mu, Weidong Zhang, Elizabeth R. Butch, Scott E. Snyder, Bing Yan

Research output: Contribution to journalArticle

189 Citations (Scopus)

Abstract

Soluble carbon nanotubes show promise as materials for in vivo delivery and imaging applications. Several reports have described the in vivo toxicity of carbon nanotubes, but their effects on male reproduction have not been examined. Here, we show that repeated intravenous injections of water-soluble multiwalled carbon nanotubes into male mice can cause reversible testis damage without affecting fertility. Nanotubes accumulated in the testes, generated oxidative stress and decreased the thickness of the seminiferous epithelium in the testis at day 15, but the damage was repaired at 60 and 90 days. The quantity, quality and integrity of the sperm and the levels of three major sex hormones were not significantly affected throughout the 90-day period. The fertility of treated male mice was unaffected; the pregnancy rate and delivery success of female mice that mated with the treated male mice did not differ from those that mated with untreated male mice.

Original languageEnglish (US)
Pages (from-to)683-689
Number of pages7
JournalNature Nanotechnology
Volume5
Issue number9
DOIs
StatePublished - Sep 2010
Externally publishedYes

Fingerprint

fertility
testes
Carbon Nanotubes
mice
Carbon nanotubes
carbon nanotubes
damage
Oxidative stress
Multiwalled carbon nanotubes (MWCN)
causes
Hormones
Gonadal Steroid Hormones
Nanotubes
Toxicity
delivery
Imaging techniques
Water
hormones
epithelium
pregnancy

ASJC Scopus subject areas

  • Bioengineering
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering
  • Materials Science(all)
  • Condensed Matter Physics
  • Electrical and Electronic Engineering

Cite this

Repeated administrations of carbon nanotubes in male mice cause reversible testis damage without affecting fertility. / Bai, Yuhong; Zhang, Yi; Zhang, Jingping; Mu, Qingxin; Zhang, Weidong; Butch, Elizabeth R.; Snyder, Scott E.; Yan, Bing.

In: Nature Nanotechnology, Vol. 5, No. 9, 09.2010, p. 683-689.

Research output: Contribution to journalArticle

Bai, Yuhong ; Zhang, Yi ; Zhang, Jingping ; Mu, Qingxin ; Zhang, Weidong ; Butch, Elizabeth R. ; Snyder, Scott E. ; Yan, Bing. / Repeated administrations of carbon nanotubes in male mice cause reversible testis damage without affecting fertility. In: Nature Nanotechnology. 2010 ; Vol. 5, No. 9. pp. 683-689.
@article{c4ade181900047248b01ab345e921ee6,
title = "Repeated administrations of carbon nanotubes in male mice cause reversible testis damage without affecting fertility",
abstract = "Soluble carbon nanotubes show promise as materials for in vivo delivery and imaging applications. Several reports have described the in vivo toxicity of carbon nanotubes, but their effects on male reproduction have not been examined. Here, we show that repeated intravenous injections of water-soluble multiwalled carbon nanotubes into male mice can cause reversible testis damage without affecting fertility. Nanotubes accumulated in the testes, generated oxidative stress and decreased the thickness of the seminiferous epithelium in the testis at day 15, but the damage was repaired at 60 and 90 days. The quantity, quality and integrity of the sperm and the levels of three major sex hormones were not significantly affected throughout the 90-day period. The fertility of treated male mice was unaffected; the pregnancy rate and delivery success of female mice that mated with the treated male mice did not differ from those that mated with untreated male mice.",
author = "Yuhong Bai and Yi Zhang and Jingping Zhang and Qingxin Mu and Weidong Zhang and Butch, {Elizabeth R.} and Snyder, {Scott E.} and Bing Yan",
year = "2010",
month = "9",
doi = "10.1038/nnano.2010.153",
language = "English (US)",
volume = "5",
pages = "683--689",
journal = "Nature Nanotechnology",
issn = "1748-3387",
publisher = "Nature Publishing Group",
number = "9",

}

TY - JOUR

T1 - Repeated administrations of carbon nanotubes in male mice cause reversible testis damage without affecting fertility

AU - Bai, Yuhong

AU - Zhang, Yi

AU - Zhang, Jingping

AU - Mu, Qingxin

AU - Zhang, Weidong

AU - Butch, Elizabeth R.

AU - Snyder, Scott E.

AU - Yan, Bing

PY - 2010/9

Y1 - 2010/9

N2 - Soluble carbon nanotubes show promise as materials for in vivo delivery and imaging applications. Several reports have described the in vivo toxicity of carbon nanotubes, but their effects on male reproduction have not been examined. Here, we show that repeated intravenous injections of water-soluble multiwalled carbon nanotubes into male mice can cause reversible testis damage without affecting fertility. Nanotubes accumulated in the testes, generated oxidative stress and decreased the thickness of the seminiferous epithelium in the testis at day 15, but the damage was repaired at 60 and 90 days. The quantity, quality and integrity of the sperm and the levels of three major sex hormones were not significantly affected throughout the 90-day period. The fertility of treated male mice was unaffected; the pregnancy rate and delivery success of female mice that mated with the treated male mice did not differ from those that mated with untreated male mice.

AB - Soluble carbon nanotubes show promise as materials for in vivo delivery and imaging applications. Several reports have described the in vivo toxicity of carbon nanotubes, but their effects on male reproduction have not been examined. Here, we show that repeated intravenous injections of water-soluble multiwalled carbon nanotubes into male mice can cause reversible testis damage without affecting fertility. Nanotubes accumulated in the testes, generated oxidative stress and decreased the thickness of the seminiferous epithelium in the testis at day 15, but the damage was repaired at 60 and 90 days. The quantity, quality and integrity of the sperm and the levels of three major sex hormones were not significantly affected throughout the 90-day period. The fertility of treated male mice was unaffected; the pregnancy rate and delivery success of female mice that mated with the treated male mice did not differ from those that mated with untreated male mice.

UR - http://www.scopus.com/inward/record.url?scp=77956442702&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77956442702&partnerID=8YFLogxK

U2 - 10.1038/nnano.2010.153

DO - 10.1038/nnano.2010.153

M3 - Article

C2 - 20693989

AN - SCOPUS:77956442702

VL - 5

SP - 683

EP - 689

JO - Nature Nanotechnology

JF - Nature Nanotechnology

SN - 1748-3387

IS - 9

ER -