Relationship of changes in pH and energy status to hypoxic cell fraction and hyperthermia sensitivity

Jason A. Koutcher, Deborah Barnett, Alice B. Kornblith, David Cowburn, Thomas J. Brady, Leo E. Gerweck

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The relative concentrations of nucleotide triphosphates, creatine phosphate, inorganic phosphate, and pH have been evaluated as a function of tumor volume in a murine fibrosarcoma (FSaII) by 31P NMR spectroscopy. As the tumor volume increased from 60-1250 mm3, the ratio of phosphocreatine to inorganic phosphate systemically decreased. This decrease paralleled a decrease in the ratio of nucleotide triphosphate to inorganic phosphate in thesame tumor volume range. The tumor pH as measured by 31P NMR decreased slightly with tumor growth. A pH of 7.17 ± 0.07 (n = 17) was found for tumors between 60 and 150 mm3, whereas for tumors greater than 900 mm3, a pH of 7.05 ± .03 (n = 6) was noted. Intermediate size tumors (151-900) had a pH of 7.12 ± 0.09 (n = 18). The change in tumor energy status with tumor volume inversely paralleled the change in tumor radiobiologic hypoxic cell fraction and suggested a causal relationship between tumor nutrient status and energy status. Tumor thermal sensitivity also increased with tumor volume, suggesting a relationship between pH, energy status, and thermal sensitivity, as has been demonstrated under in vitro conditions. Each NMR parameter was found to correlate significantly with tumor volume independent of the other NMR parameters.

Original languageEnglish (US)
Pages (from-to)1429-1435
Number of pages7
JournalInternational Journal of Radiation Oncology, Biology, Physics
Volume18
Issue number6
DOIs
StatePublished - 1990
Externally publishedYes

Fingerprint

hyperthermia
Fever
tumors
Tumor Burden
sensitivity
Neoplasms
energy
Phosphocreatine
Phosphates
phosphates
Nucleotides
Hot Temperature
nuclear magnetic resonance
nucleotides
Fibrosarcoma
creatine
Magnetic Resonance Spectroscopy
nutrients
Food

Keywords

  • P-nuclear magnetic resonance
  • Energy status
  • Heat sensitivity
  • Tumor hypoxia

ASJC Scopus subject areas

  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Radiation

Cite this

Relationship of changes in pH and energy status to hypoxic cell fraction and hyperthermia sensitivity. / Koutcher, Jason A.; Barnett, Deborah; Kornblith, Alice B.; Cowburn, David; Brady, Thomas J.; Gerweck, Leo E.

In: International Journal of Radiation Oncology, Biology, Physics, Vol. 18, No. 6, 1990, p. 1429-1435.

Research output: Contribution to journalArticle

Koutcher, Jason A. ; Barnett, Deborah ; Kornblith, Alice B. ; Cowburn, David ; Brady, Thomas J. ; Gerweck, Leo E. / Relationship of changes in pH and energy status to hypoxic cell fraction and hyperthermia sensitivity. In: International Journal of Radiation Oncology, Biology, Physics. 1990 ; Vol. 18, No. 6. pp. 1429-1435.
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