TY - JOUR
T1 - Relation between natural killer cells and neoplastic cells in serous fluids
AU - Laurini, Javier A.
AU - Garcia, Alejandro
AU - Elsner, Boris
AU - Bellotti, Marta
AU - Rescia, Carla
PY - 2000/6
Y1 - 2000/6
N2 - Natural killer (NK) cells are capable of lysing certain tumor cells in vitro. We investigated whether the percentage of NK cells (NK%) in serous effusions has any relation with the presence of malignant cells. Fresh effusions were routinely processed, and a portion of them was submitted for lymphocyte immunophenotyping, using a flow cytometer. Fifty-one fluids (15 peritoneal, 36 pleural), from 47 patients were analyzed. Thirty-two cases were negative and 19 were positive for neoplastic cells: there were 13 adenocarcinomas, 2 malignant mesotheliomas (MM), 3 malignant lymphomas (ML), and 1 rhabdomyosarcoma (R). Negative cases showed an average NK% of 5.09% (1- 16%). Effusions positive for adenocarcinoma had an average NK% of 22.08% (12- 33%) (P < 0.001). MM, ML, and R had a low NK%, with an average of 3.01% (0- 5%). Investigation of the NK% could be useful in cytologically doubtful cases and in the differential diagnosis between MM and adenocarcinoma. (C) 2000 Wiley-Liss, Inc.
AB - Natural killer (NK) cells are capable of lysing certain tumor cells in vitro. We investigated whether the percentage of NK cells (NK%) in serous effusions has any relation with the presence of malignant cells. Fresh effusions were routinely processed, and a portion of them was submitted for lymphocyte immunophenotyping, using a flow cytometer. Fifty-one fluids (15 peritoneal, 36 pleural), from 47 patients were analyzed. Thirty-two cases were negative and 19 were positive for neoplastic cells: there were 13 adenocarcinomas, 2 malignant mesotheliomas (MM), 3 malignant lymphomas (ML), and 1 rhabdomyosarcoma (R). Negative cases showed an average NK% of 5.09% (1- 16%). Effusions positive for adenocarcinoma had an average NK% of 22.08% (12- 33%) (P < 0.001). MM, ML, and R had a low NK%, with an average of 3.01% (0- 5%). Investigation of the NK% could be useful in cytologically doubtful cases and in the differential diagnosis between MM and adenocarcinoma. (C) 2000 Wiley-Liss, Inc.
KW - Effusions
KW - Flow cytometry
KW - Immunophenotyping
KW - Natural killer cells
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U2 - 10.1002/(SICI)1097-0339(200006)22:6<347::AID-DC4>3.0.CO;2-T
DO - 10.1002/(SICI)1097-0339(200006)22:6<347::AID-DC4>3.0.CO;2-T
M3 - Article
C2 - 10820527
AN - SCOPUS:0034094112
SN - 8755-1039
VL - 22
SP - 347
EP - 350
JO - Diagnostic Cytopathology
JF - Diagnostic Cytopathology
IS - 6
ER -