Regulatory variants for the expression of H-2 antigens. I. Isolation and characterization

T. V. Rajan, S. G. Nathenson, Matthew D. Scharff

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17 Citations (Scopus)

Abstract

By subjecting 'mutagenized' mouse myeloma cells repeatedly to selection with antiserum against H-2Kd, variants were isolated that expressed little or no H-2Kd alloantigens. The phenotype was unstable, with the culture accumulating revertants in the absence of selective pressure. The variants not only failed to express the selected antigen, but also the antigen coded by the closely linked H-2Dd gene. The cells reexpressed this antigen with the same kinetics as for the selected antigen. Several physiologic parameters (karyotype, cell morphology, size, and growth curves) were not altered by the loss of H-2 antigens. However, when injected iv, the H-2 deficient cells did not lodge in the spleen as readily as did the wild type cells.

Original languageEnglish (US)
Pages (from-to)1221-1227
Number of pages7
JournalJournal of the National Cancer Institute
Volume56
Issue number6
StatePublished - 1976

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H-2 Antigens
Antigens
Cell Enlargement
Isoantigens
Karyotype
Immune Sera
Spleen
Phenotype
Genes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Regulatory variants for the expression of H-2 antigens. I. Isolation and characterization. / Rajan, T. V.; Nathenson, S. G.; Scharff, Matthew D.

In: Journal of the National Cancer Institute, Vol. 56, No. 6, 1976, p. 1221-1227.

Research output: Contribution to journalArticle

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