TY - JOUR
T1 - Regulatory variants for the expression of h-2 antigens. i. isolation and characterization
AU - Rajan, T. V.
AU - Nathenson, Stanley G.
AU - Scharff, Matthew D.
PY - 1976/6
Y1 - 1976/6
N2 - By subjecting “mutagenized” mouse myeloma cells repeatedly to selection with antiserum against H-2Kd, we isolated variants that expressed little or no H-2Kd alloantigens. The phenotype was unstable, with the culture accumulating revertants in the absence of selective pressure. The variants not only failed to express the selected antigen, but also the antigen coded by the closely linked H-2Dd gene. The cells reexpressed this antigen with the same kinetics as for the selected antigen. Several physiologic parameters (karyotype, cell morphology, size, and growth curves) were not altered by the loss of H-2 antigens. However, when injected iv, the H-2-deficient cells did not lodge in the spleen as readily as did the wild-type cell.
AB - By subjecting “mutagenized” mouse myeloma cells repeatedly to selection with antiserum against H-2Kd, we isolated variants that expressed little or no H-2Kd alloantigens. The phenotype was unstable, with the culture accumulating revertants in the absence of selective pressure. The variants not only failed to express the selected antigen, but also the antigen coded by the closely linked H-2Dd gene. The cells reexpressed this antigen with the same kinetics as for the selected antigen. Several physiologic parameters (karyotype, cell morphology, size, and growth curves) were not altered by the loss of H-2 antigens. However, when injected iv, the H-2-deficient cells did not lodge in the spleen as readily as did the wild-type cell.
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U2 - 10.1093/jnci/56.6.1221
DO - 10.1093/jnci/56.6.1221
M3 - Article
C2 - 994223
AN - SCOPUS:0017074266
SN - 0027-8874
VL - 56
SP - 1221
EP - 1227
JO - Journal of the National Cancer Institute
JF - Journal of the National Cancer Institute
IS - 6
ER -