Regulatory variants for the expression of h-2 antigens. i. isolation and characterization

T. V. Rajan, Stanley G. Nathenson, Matthew D. Scharff

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Abstract

By subjecting “mutagenized” mouse myeloma cells repeatedly to selection with antiserum against H-2Kd, we isolated variants that expressed little or no H-2Kd alloantigens. The phenotype was unstable, with the culture accumulating revertants in the absence of selective pressure. The variants not only failed to express the selected antigen, but also the antigen coded by the closely linked H-2Dd gene. The cells reexpressed this antigen with the same kinetics as for the selected antigen. Several physiologic parameters (karyotype, cell morphology, size, and growth curves) were not altered by the loss of H-2 antigens. However, when injected iv, the H-2-deficient cells did not lodge in the spleen as readily as did the wild-type cell.

Original languageEnglish (US)
Pages (from-to)1221-1227
Number of pages7
JournalJournal of the National Cancer Institute
Volume56
Issue number6
DOIs
Publication statusPublished - Jun 1976

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ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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