Regulatory effects of IL-18 on cytokine profiles and development of myocarditis during Trypanosoma cruzi infection

Lísia Esper, Lara Utsch, Frederico M. Soriani, Fátima Brant, Rosa Maria Esteves Arantes, Camila F. Campos, Vanessa Pinho, Danielle G. Souza, Mauro Martins Teixeira, Herbert Bernard Tanowitz, Leda Quercia Vieira, Fabiana Simão Machado

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Chagas disease, caused by Trypanosoma cruzi (Tc), is an important cause of heart disease. Resistance to Tc infection is multifactorial and associated with Th1 response. IL-18 plays an important role in regulation of IFN-γ production/development of Th1 response. However, the role of IL-18 in the setting of Tc infection remains unclear. Therefore, we investigated the role of IL-18 in the modulation of immune response and myocarditis in Tc infection. C57BL/6 and IL-18 KO mice were infected with Tc (Y or Colombian strain) and parasitemia, immune response and pathology were evaluated. Y strain infection of IL-18 KO did not alter any parameters when compared with C57BL/6 mice. However, during the acute phase (20 and 40 days post infection-dpi), Colombian strain infected-IL-18 KO mice displayed higher serum levels of IL-12 and IFN-γ, respectively, and at the chronic phase (100dpi) an increase in splenic IFN-γ-producing CD4+ and CD8+ T memory cells. There was an IL-10, FOXP3 and CD4+CD25+ cells reduction during acute infection in spleen. Additionally, there was a significant reduction in leukocyte infiltration and parasite load in myocardium of chronically infected IL-18 KO mice. Collectively, these data indicate that IL-18 contributes to the pathogenesis of Tc-induced myocarditis when infected with Colombian but not Y strain. These observations also underscore that parasite and host strain differences are important in evaluation of experimental Tc infection pathogenesis.

Original languageEnglish (US)
Pages (from-to)481-490
Number of pages10
JournalMicrobes and Infection
Volume16
Issue number6
DOIs
StatePublished - 2014

Fingerprint

Interleukin-18
Trypanosoma cruzi
Myocarditis
Cytokines
Infection
Parasite Load
Parasitemia
Chagas Disease
Interleukin-12
Inbred C57BL Mouse
Interleukin-10
Heart Diseases
Myocardium
Parasites
Leukocytes
Spleen
Pathology
Serum

Keywords

  • Cytokine
  • IL-18
  • Myocarditis
  • Trypanosoma cruzi

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Infectious Diseases
  • Medicine(all)

Cite this

Esper, L., Utsch, L., Soriani, F. M., Brant, F., Esteves Arantes, R. M., Campos, C. F., ... Machado, F. S. (2014). Regulatory effects of IL-18 on cytokine profiles and development of myocarditis during Trypanosoma cruzi infection. Microbes and Infection, 16(6), 481-490. https://doi.org/10.1016/j.micinf.2014.03.007

Regulatory effects of IL-18 on cytokine profiles and development of myocarditis during Trypanosoma cruzi infection. / Esper, Lísia; Utsch, Lara; Soriani, Frederico M.; Brant, Fátima; Esteves Arantes, Rosa Maria; Campos, Camila F.; Pinho, Vanessa; Souza, Danielle G.; Teixeira, Mauro Martins; Tanowitz, Herbert Bernard; Vieira, Leda Quercia; Machado, Fabiana Simão.

In: Microbes and Infection, Vol. 16, No. 6, 2014, p. 481-490.

Research output: Contribution to journalArticle

Esper, L, Utsch, L, Soriani, FM, Brant, F, Esteves Arantes, RM, Campos, CF, Pinho, V, Souza, DG, Teixeira, MM, Tanowitz, HB, Vieira, LQ & Machado, FS 2014, 'Regulatory effects of IL-18 on cytokine profiles and development of myocarditis during Trypanosoma cruzi infection', Microbes and Infection, vol. 16, no. 6, pp. 481-490. https://doi.org/10.1016/j.micinf.2014.03.007
Esper, Lísia ; Utsch, Lara ; Soriani, Frederico M. ; Brant, Fátima ; Esteves Arantes, Rosa Maria ; Campos, Camila F. ; Pinho, Vanessa ; Souza, Danielle G. ; Teixeira, Mauro Martins ; Tanowitz, Herbert Bernard ; Vieira, Leda Quercia ; Machado, Fabiana Simão. / Regulatory effects of IL-18 on cytokine profiles and development of myocarditis during Trypanosoma cruzi infection. In: Microbes and Infection. 2014 ; Vol. 16, No. 6. pp. 481-490.
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abstract = "Chagas disease, caused by Trypanosoma cruzi (Tc), is an important cause of heart disease. Resistance to Tc infection is multifactorial and associated with Th1 response. IL-18 plays an important role in regulation of IFN-γ production/development of Th1 response. However, the role of IL-18 in the setting of Tc infection remains unclear. Therefore, we investigated the role of IL-18 in the modulation of immune response and myocarditis in Tc infection. C57BL/6 and IL-18 KO mice were infected with Tc (Y or Colombian strain) and parasitemia, immune response and pathology were evaluated. Y strain infection of IL-18 KO did not alter any parameters when compared with C57BL/6 mice. However, during the acute phase (20 and 40 days post infection-dpi), Colombian strain infected-IL-18 KO mice displayed higher serum levels of IL-12 and IFN-γ, respectively, and at the chronic phase (100dpi) an increase in splenic IFN-γ-producing CD4+ and CD8+ T memory cells. There was an IL-10, FOXP3 and CD4+CD25+ cells reduction during acute infection in spleen. Additionally, there was a significant reduction in leukocyte infiltration and parasite load in myocardium of chronically infected IL-18 KO mice. Collectively, these data indicate that IL-18 contributes to the pathogenesis of Tc-induced myocarditis when infected with Colombian but not Y strain. These observations also underscore that parasite and host strain differences are important in evaluation of experimental Tc infection pathogenesis.",
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AU - Esteves Arantes, Rosa Maria

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AU - Pinho, Vanessa

AU - Souza, Danielle G.

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AU - Tanowitz, Herbert Bernard

AU - Vieira, Leda Quercia

AU - Machado, Fabiana Simão

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