Abstract
In the CNS, astrocytes are significant sources of RANTES/CCL5 (regulated upon activation, normal T cell expressed and secreted), a CC-chemokine with important biological function. Astrocyte RANTES/CCL5 has been shown to be induced by interleukin-1 (IL-1), with interferon-γ (IFNγ) as a primer, but whether type I interferons play any role in the expression of RANTES/CCL5 is not known. In this report, we studied the detailed mechanism of RANTES/CCL5 induction in primary human astrocytes activated with IL-1 and IFNβ. Ribonuclease protection assay and ELISA showed that IFNβ, although not effective alone, increased IL-1-induced RANTES/CCL5 expression, but did not antagonize IFNγ. IL-1 or IL-1/IFNβ-induced RANTES/CCL5 expression was inhibited by the super-repressor IκBα or inhibitors of p38 or c-Jun N-terminal kinase (JNK) MAPKs (mitogen-activated protein kinases), but not by extracellular signal regulated kinases (ERK) inhibitors. IFNβ enhanced IL-1-induced phosphorylation of p38 MAPK, but was not effective alone. Transfection with mutated RANTES/CCL5 promoter-reporter constructs revealed that κB, interferon-stimulated response element (ISRE) and CAATT-enhancer binding protein-β (C/EBPβ) sites all contributed to IL-1/IFNβ-induced RANTES/CCL5 transcription. IFNβ synergized with IL-1 to induce nuclear accumulation of C/EBPβ protein. They also synergized to form nuclear ISRE complexes with Stat1, Stat2 and interferon regulatory factor-1 (IRF-1) proteins. Together, our results demonstrate that IFNβ plays a positive regulatory role in the expression of RANTES/CCL5 in human astrocytes through several distinct mechanisms.
Original language | English (US) |
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Pages (from-to) | 297-308 |
Number of pages | 12 |
Journal | Journal of Neurochemistry |
Volume | 90 |
Issue number | 2 |
DOIs | |
State | Published - Jul 2004 |
Keywords
- Brain
- Chemokine
- Human
- Promoter
- Transcription
ASJC Scopus subject areas
- Biochemistry
- Cellular and Molecular Neuroscience